Assay Detail
Binding
CHEMBL4183550
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Inhibition of NS5A in HuH7 cell infected HCV genotype 1a H77 assessed as decrease in viral replication after 3 days by luciferase reporter gene assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Hepatitis C virus (isolate H77) |
| Confidence | 8 — Homologous single protein target |
| Curated By | Intermediate |
Publication
Highlights of the Structure-Activity Relationships of Benzimidazole Linked Pyrrolidines Leading to the Discovery of the Hepatitis C Virus NS5A Inhibitor Pibrentasvir (ABT-530).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 25 | 10.29 | 10.92 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4203054 | — | — | 1 | 10.92 |
| CHEMBL4203574 | — | — | 1 | 10.92 |
| CHEMBL3127326 | OMBITASVIR | 4.0 | 1 | 10.85 |
| CHEMBL4208073 | — | — | 1 | 10.74 |
| CHEMBL4211348 | — | — | 1 | 10.72 |
| CHEMBL4203254 | — | — | 1 | 10.42 |
| CHEMBL4215679 | — | — | 1 | 10.15 |
| CHEMBL3127327 | — | — | 1 | 9.87 |
| CHEMBL4202982 | — | — | 1 | 9.72 |
| CHEMBL4208591 | — | — | 1 | 9.49 |
| CHEMBL4206465 | — | — | 1 | 9.42 |
| CHEMBL4215187 | — | — | 1 | - |
| CHEMBL4205352 | — | — | 1 | - |
| CHEMBL4210254 | — | — | 1 | - |
| CHEMBL4218872 | — | — | 1 | - |
| CHEMBL3960757 | — | — | 1 | - |
| CHEMBL4211486 | — | — | 1 | - |
| CHEMBL4215241 | — | — | 1 | - |
| CHEMBL4204257 | — | — | 1 | - |
| CHEMBL4215254 | — | — | 1 | - |
| CHEMBL4202479 | — | — | 1 | - |
| CHEMBL4215923 | — | — | 1 | - |
| CHEMBL4203712 | — | — | 1 | - |
| CHEMBL3545123 | PIBRENTASVIR | 4.0 | 1 | - |
| CHEMBL3915742 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4203054 | — | EC50 | = | 0.012 | nM | 10.92 |
| CHEMBL4203574 | — | EC50 | = | 0.012 | nM | 10.92 |
| CHEMBL3127326 | OMBITASVIR | EC50 | = | 0.014 | nM | 10.85 |
| CHEMBL4208073 | — | EC50 | = | 0.018 | nM | 10.74 |
| CHEMBL4211348 | — | EC50 | = | 0.019 | nM | 10.72 |
| CHEMBL4203254 | — | EC50 | = | 0.038 | nM | 10.42 |
| CHEMBL4215679 | — | EC50 | = | 0.071 | nM | 10.15 |
| CHEMBL3127327 | — | EC50 | = | 0.136 | nM | 9.87 |
| CHEMBL4202982 | — | EC50 | = | 0.189 | nM | 9.72 |
| CHEMBL4208591 | — | EC50 | = | 0.324 | nM | 9.49 |
| CHEMBL4206465 | — | EC50 | = | 0.379 | nM | 9.42 |
| CHEMBL4215187 | — | EC50 | = | 0.003 | nM | - |
| CHEMBL4205352 | — | EC50 | = | 0.001 | nM | - |
| CHEMBL4210254 | — | EC50 | = | 0.004 | nM | - |
| CHEMBL4218872 | — | EC50 | = | 0.001 | nM | - |
| CHEMBL3960757 | — | EC50 | = | 0.009 | nM | - |
| CHEMBL4211486 | — | EC50 | = | 0.002 | nM | - |
| CHEMBL4215241 | — | EC50 | = | 0.006 | nM | - |
| CHEMBL4204257 | — | EC50 | = | 0.003 | nM | - |
| CHEMBL4215254 | — | EC50 | = | 0.004 | nM | - |
| CHEMBL4202479 | — | EC50 | = | 0.004 | nM | - |
| CHEMBL4215923 | — | EC50 | = | 0.002 | nM | - |
| CHEMBL4203712 | — | EC50 | = | 0.005 | nM | - |
| CHEMBL3545123 | PIBRENTASVIR | EC50 | = | 0.0018 | nM | - |
| CHEMBL3915742 | — | EC50 | = | 0.005 | nM | - |