Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4183554

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HCV genotype 1b Con1 NS5A expressed in HuH7 cell infected HCV genotype 2a assessed as decrease in viral replication after 3 days by luciferase reporter gene assay
ChEMBL ↗
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus (isolate Con1)
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Genome polyprotein (CHEMBL5536) ↗
Type SINGLE PROTEIN
Organism Hepatitis C virus genotype 1b (isolate Con1) (HCV)

Publication

Highlights of the Structure-Activity Relationships of Benzimidazole Linked Pyrrolidines Leading to the Discovery of the Hepatitis C Virus NS5A Inhibitor Pibrentasvir (ABT-530).
Wagner R, Randolph JT, Patel SV, Nelson L, Matulenko MA, Keddy R, Pratt JK, Liu D, Krueger AC, Donner PL, Hutchinson DK, Flentge C, Betebenner D, Rockway T, Maring CJ, Ng TI, Krishnan P, Pilot-Matias T, Collins C, Panchal N, Reisch T, Dekhtyar T, Mondal R, Stolarik DF, Gao Y, Gao W, Beno DA, Kati WM.
J Med Chem (2018) 61 : 4052 -4066
PubMed 29653491 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 25 10.39 11.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4215254 — — 1 11.00
CHEMBL4202479 — — 1 10.96
CHEMBL3127326 OMBITASVIR 4.0 1 10.92
CHEMBL4215241 — — 1 10.92
CHEMBL4203054 — — 1 10.92
CHEMBL3915742 — — 1 10.89
CHEMBL4204257 — — 1 10.70
CHEMBL4203254 — — 1 10.55
CHEMBL4203574 — — 1 10.39
CHEMBL4211348 — — 1 10.37
CHEMBL4203712 — — 1 10.36
CHEMBL4208073 — — 1 10.32
CHEMBL3960757 — — 1 9.82
CHEMBL4202982 — — 1 9.51
CHEMBL4208591 — — 1 9.33
CHEMBL4206465 — — 1 9.22
CHEMBL4215923 — — 1 -
CHEMBL3127327 — — 1 -
CHEMBL4211486 — — 1 -
CHEMBL4218872 — — 1 -
CHEMBL4210254 — — 1 -
CHEMBL4205352 — — 1 -
CHEMBL4215187 — — 1 -
CHEMBL3545123 PIBRENTASVIR 4.0 1 -
CHEMBL4215679 — — 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4215254 — EC50 = 0.01 nM 11.00
CHEMBL4202479 — EC50 = 0.011 nM 10.96
CHEMBL3127326 OMBITASVIR EC50 = 0.012 nM 10.92
CHEMBL4215241 — EC50 = 0.012 nM 10.92
CHEMBL4203054 — EC50 = 0.012 nM 10.92
CHEMBL3915742 — EC50 = 0.013 nM 10.89
CHEMBL4204257 — EC50 = 0.02 nM 10.70
CHEMBL4203254 — EC50 = 0.028 nM 10.55
CHEMBL4203574 — EC50 = 0.041 nM 10.39
CHEMBL4211348 — EC50 = 0.043 nM 10.37
CHEMBL4203712 — EC50 = 0.044 nM 10.36
CHEMBL4208073 — EC50 = 0.048 nM 10.32
CHEMBL3960757 — EC50 = 0.152 nM 9.82
CHEMBL4202982 — EC50 = 0.307 nM 9.51
CHEMBL4208591 — EC50 = 0.468 nM 9.33
CHEMBL4206465 — EC50 = 0.598 nM 9.22
CHEMBL4215923 — EC50 = 0.008 nM -
CHEMBL3127327 — EC50 - — -
CHEMBL4211486 — EC50 = 0.008 nM -
CHEMBL4218872 — EC50 = 0.003 nM -
CHEMBL4210254 — EC50 = 0.009 nM -
CHEMBL4205352 — EC50 = 0.003 nM -
CHEMBL4215187 — EC50 = 0.008 nM -
CHEMBL3545123 PIBRENTASVIR EC50 = 0.0023 nM -
CHEMBL4215679 — EC50 - — -