Assay Detail
Binding
CHEMBL4183557
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of HCV genotype 1b Con1 NS5A expressed in HuH7 cell infected HCV genotype 4a assessed as decrease in viral replication after 3 days by luciferase reporter gene assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Hepatitis C virus (isolate Con1) |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Genome polyprotein (CHEMBL5536) ↗| Type | SINGLE PROTEIN |
| Organism | Hepatitis C virus genotype 1b (isolate Con1) (HCV) |
Publication
Highlights of the Structure-Activity Relationships of Benzimidazole Linked Pyrrolidines Leading to the Discovery of the Hepatitis C Virus NS5A Inhibitor Pibrentasvir (ABT-530).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 25 | 10.30 | 10.89 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4211348 | — | — | 1 | 10.89 |
| CHEMBL4203254 | — | — | 1 | 10.85 |
| CHEMBL4208073 | — | — | 1 | 10.77 |
| CHEMBL4203574 | — | — | 1 | 10.70 |
| CHEMBL4202982 | — | — | 1 | 9.77 |
| CHEMBL4208591 | — | — | 1 | 9.61 |
| CHEMBL4206465 | — | — | 1 | 9.50 |
| CHEMBL3960757 | — | — | 1 | - |
| CHEMBL4215254 | — | — | 1 | - |
| CHEMBL4202479 | — | — | 1 | - |
| CHEMBL4203054 | — | — | 1 | - |
| CHEMBL4215679 | — | — | 1 | - |
| CHEMBL3127327 | — | — | 1 | - |
| CHEMBL3915742 | — | — | 1 | - |
| CHEMBL4203712 | — | — | 1 | - |
| CHEMBL4215923 | — | — | 1 | - |
| CHEMBL4204257 | — | — | 1 | - |
| CHEMBL4211486 | — | — | 1 | - |
| CHEMBL4218872 | — | — | 1 | - |
| CHEMBL4210254 | — | — | 1 | - |
| CHEMBL4205352 | — | — | 1 | - |
| CHEMBL4215187 | — | — | 1 | - |
| CHEMBL3545123 | PIBRENTASVIR | 4.0 | 1 | - |
| CHEMBL4215241 | — | — | 1 | - |
| CHEMBL3127326 | OMBITASVIR | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4211348 | — | EC50 | = | 0.013 | nM | 10.89 |
| CHEMBL4203254 | — | EC50 | = | 0.014 | nM | 10.85 |
| CHEMBL4208073 | — | EC50 | = | 0.017 | nM | 10.77 |
| CHEMBL4203574 | — | EC50 | = | 0.02 | nM | 10.70 |
| CHEMBL4202982 | — | EC50 | = | 0.17 | nM | 9.77 |
| CHEMBL4208591 | — | EC50 | = | 0.243 | nM | 9.61 |
| CHEMBL4206465 | — | EC50 | = | 0.316 | nM | 9.50 |
| CHEMBL3960757 | — | EC50 | = | 0.006 | nM | - |
| CHEMBL4215254 | — | EC50 | = | 0.009 | nM | - |
| CHEMBL4202479 | — | EC50 | = | 0.006 | nM | - |
| CHEMBL4203054 | — | EC50 | = | 0.005 | nM | - |
| CHEMBL4215679 | — | EC50 | - | — | - | |
| CHEMBL3127327 | — | EC50 | - | — | - | |
| CHEMBL3915742 | — | EC50 | = | 0.005 | nM | - |
| CHEMBL4203712 | — | EC50 | = | 0.006 | nM | - |
| CHEMBL4215923 | — | EC50 | = | 0.004 | nM | - |
| CHEMBL4204257 | — | EC50 | = | 0.009 | nM | - |
| CHEMBL4211486 | — | EC50 | = | 0.004 | nM | - |
| CHEMBL4218872 | — | EC50 | = | 0.001 | nM | - |
| CHEMBL4210254 | — | EC50 | = | 0.006 | nM | - |
| CHEMBL4205352 | — | EC50 | = | 0.003 | nM | - |
| CHEMBL4215187 | — | EC50 | = | 0.006 | nM | - |
| CHEMBL3545123 | PIBRENTASVIR | EC50 | = | 0.0019 | nM | - |
| CHEMBL4215241 | — | EC50 | = | 0.005 | nM | - |
| CHEMBL3127326 | OMBITASVIR | EC50 | = | 0.0017 | nM | - |