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Assay Detail

CHEMBL4183750

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Binding
Inhibition of full length Trypanosoma brucei PDEB1 expressed in Sf21 insect cells using cAMP as substrate by scintillation proximity assay
26
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Trypanosoma brucei
Cell Type Sf21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Class 1 phosphodiesterase PDEB1 (CHEMBL2010636)
Type SINGLE PROTEIN
Organism Trypanosoma brucei

Publication

Targeting a Subpocket in Trypanosoma brucei Phosphodiesterase B1 (TbrPDEB1) Enables the Structure-Based Discovery of Selective Inhibitors with Trypanocidal Activity.
Blaazer AR, Singh AK, de Heuvel E, Edink E, Orrling KM, Veerman JJN, van den Bergh T, Jansen C, Balasubramaniam E, Mooij WJ, Custers H, Sijm M, Tagoe DNA, Kalejaiye TD, Munday JC, Tenor H, Matheeussen A, Wijtmans M, Siderius M, de Graaf C, Maes L, de Koning HP, Bailey DS, Sterk GJ, de Esch IJP, Brown DG, Leurs R.
J Med Chem (2018) 61 : 3870 -3888
PubMed 29672041 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 26 6.33 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2326941 1 8.40
CHEMBL4210475 2 7.40
CHEMBL4204999 1 7.00
CHEMBL4202975 1 6.80
CHEMBL4208363 1 6.70
CHEMBL4218658 1 6.60
CHEMBL4214364 1 6.50
CHEMBL4204966 1 6.40
CHEMBL4206114 1 6.40
CHEMBL4217833 1 6.40
CHEMBL4209841 1 6.30
CHEMBL4209331 1 6.30
CHEMBL4202704 1 6.30
CHEMBL4213296 1 6.30
CHEMBL4216582 1 6.20
CHEMBL4218119 1 6.20
CHEMBL4218358 1 6.10
CHEMBL4214240 1 6.10
CHEMBL4213729 1 6.00
CHEMBL4206395 1 5.80
CHEMBL4205916 1 5.80
CHEMBL4213432 1 5.70
CHEMBL193240 ROFLUMILAST 4.0 1 5.50
CHEMBL4209972 1 5.40
CHEMBL4203531 1 5.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2326941 Ki = 3.981 nM 8.40
CHEMBL4210475 Ki = 39.81 nM 7.40
CHEMBL4210475 Ki = 100.0 nM 7.00
CHEMBL4204999 Ki = 100.0 nM 7.00
CHEMBL4202975 Ki = 158.49 nM 6.80
CHEMBL4208363 Ki = 199.53 nM 6.70
CHEMBL4218658 Ki = 251.19 nM 6.60
CHEMBL4214364 Ki = 316.23 nM 6.50
CHEMBL4204966 Ki = 398.11 nM 6.40
CHEMBL4217833 Ki = 398.11 nM 6.40
CHEMBL4206114 Ki = 398.11 nM 6.40
CHEMBL4209841 Ki = 501.19 nM 6.30
CHEMBL4209331 Ki = 501.19 nM 6.30
CHEMBL4202704 Ki = 501.19 nM 6.30
CHEMBL4213296 Ki = 501.19 nM 6.30
CHEMBL4216582 Ki = 630.96 nM 6.20
CHEMBL4218119 Ki = 630.96 nM 6.20
CHEMBL4218358 Ki = 794.33 nM 6.10
CHEMBL4214240 Ki = 794.33 nM 6.10
CHEMBL4213729 Ki = 1000.0 nM 6.00
CHEMBL4206395 Ki = 1584.89 nM 5.80
CHEMBL4205916 Ki = 1584.89 nM 5.80
CHEMBL4213432 Ki = 1995.26 nM 5.70
CHEMBL193240 ROFLUMILAST Ki = 3162.28 nM 5.50
CHEMBL4209972 Ki = 3981.07 nM 5.40
CHEMBL4203531 Ki = 7943.28 nM 5.10