Assay Detail
Binding
CHEMBL4184624
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [32P]S1P from recombinant human S1PR2 expressed in Chem1 cell membranes co-expressing Galpha15 pretreated for 30 mins followed by [32P]S1P addition measured after 60 mins by scintillation counting method
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Design and synthesis of pyrazolopyridine derivatives as sphingosine 1-phosphate receptor 2 ligands.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 13 | 7.62 | 8.44 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL225155 | — | — | 1 | 8.44 |
| CHEMBL4204756 | — | — | 1 | 7.54 |
| CHEMBL4212397 | — | — | 1 | 7.25 |
| CHEMBL1368758 | — | — | 1 | 7.23 |
| CHEMBL4208671 | — | — | 1 | - |
| CHEMBL4212287 | — | — | 1 | - |
| CHEMBL4211214 | — | — | 1 | - |
| CHEMBL4205433 | — | — | 1 | - |
| CHEMBL4211376 | — | — | 1 | - |
| CHEMBL4215162 | — | — | 1 | - |
| CHEMBL4209790 | — | — | 1 | - |
| CHEMBL4213546 | — | — | 1 | - |
| CHEMBL4203070 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL225155 | — | IC50 | = | 3.6 | nM | 8.44 |
| CHEMBL4204756 | — | IC50 | = | 29.1 | nM | 7.54 |
| CHEMBL4212397 | — | IC50 | = | 56.5 | nM | 7.25 |
| CHEMBL1368758 | — | IC50 | = | 58.4 | nM | 7.23 |
| CHEMBL4208671 | — | IC50 | > | 1000.0 | nM | - |
| CHEMBL4212287 | — | IC50 | > | 1000.0 | nM | - |
| CHEMBL4211214 | — | IC50 | > | 1000.0 | nM | - |
| CHEMBL4205433 | — | IC50 | > | 1000.0 | nM | - |
| CHEMBL4211376 | — | IC50 | > | 1000.0 | nM | - |
| CHEMBL4215162 | — | IC50 | > | 1000.0 | nM | - |
| CHEMBL4209790 | — | IC50 | > | 1000.0 | nM | - |
| CHEMBL4213546 | — | IC50 | > | 1000.0 | nM | - |
| CHEMBL4203070 | — | IC50 | > | 1000.0 | nM | - |