Assay Detail
Binding
CHEMBL4184878
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of full length recombinant C-terminal His8-tagged human NAMPT using 15N-CONH2Nicotinamide as substrate preincubated for 15 mins followed by substrate addition measured after 90 mins by mass spectrometric analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Nicotinamide phosphoribosyltransferase (CHEMBL1744525) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Structure based design of nicotinamide phosphoribosyltransferase (NAMPT) inhibitors from a phenotypic screen.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 8.12 | 8.51 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4206641 | — | — | 1 | 8.51 |
| CHEMBL4213004 | — | — | 1 | 8.37 |
| CHEMBL4211993 | — | — | 1 | 8.00 |
| CHEMBL4215088 | — | — | 1 | 7.60 |
| CHEMBL4205098 | — | — | 1 | - |
| CHEMBL4215893 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4206641 | — | IC50 | = | 3.1 | nM | 8.51 |
| CHEMBL4213004 | — | IC50 | = | 4.3 | nM | 8.37 |
| CHEMBL4211993 | — | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL4215088 | — | IC50 | = | 25.0 | nM | 7.60 |
| CHEMBL4205098 | — | IC50 | < | 1.0 | nM | - |
| CHEMBL4215893 | — | IC50 | > | 2500.0 | nM | - |