Assay Detail
Binding
CHEMBL4185147
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of N-terminal hexa-His tagged human APE1 expressed in Escherichia coli BL21 (Rosetta) using fluorescein-dabcyl-containing oligonucleotide as substrate measured over 5 mins by high-throughput fluorescence assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
DNA repair nuclease/redox regulator APEX1 (CHEMBL5619) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Inhibitors of nuclease and redox activity of apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APE1/Ref-1).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 5.75 | 5.80 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2349528 | — | — | 1 | 5.80 |
| CHEMBL1443537 | — | — | 1 | 5.77 |
| CHEMBL1453126 | — | — | 1 | 5.68 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2349528 | — | IC50 | = | 1600.0 | nM | 5.80 |
| CHEMBL1443537 | — | IC50 | = | 1700.0 | nM | 5.77 |
| CHEMBL1453126 | — | IC50 | = | 2100.0 | nM | 5.68 |