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Assay Detail

CHEMBL4185153

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Binding
Inhibition of human APE1 preincubated for 15 mins followed by substrate addition by HTS assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

DNA repair nuclease/redox regulator APEX1 (CHEMBL5619)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibitors of nuclease and redox activity of apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APE1/Ref-1).
Laev SS, Salakhutdinov NF, Lavrik OI.
Bioorg Med Chem (2017) 25 : 2531 -2544
PubMed 28161249 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.48 7.26

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL275938 AURINTRICARBOXYLIC ACID 1 7.26
CHEMBL109037 1 6.96
CHEMBL1365553 1 6.60
CHEMBL56393 1 6.55
CHEMBL164 MYRICETIN 1 6.50
CHEMBL1256623 1 6.30
CHEMBL399043 THIOLACTOMYCIN 1 6.00
CHEMBL1417019 MITOXANTRONE HYDROCHLORIDE 4.0 1 5.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL275938 AURINTRICARBOXYLIC ACID IC50 = 55.0 nM 7.26
CHEMBL109037 IC50 = 110.0 nM 6.96
CHEMBL1365553 IC50 = 250.0 nM 6.60
CHEMBL56393 IC50 = 280.0 nM 6.55
CHEMBL164 MYRICETIN IC50 = 320.0 nM 6.50
CHEMBL1256623 IC50 = 500.0 nM 6.30
CHEMBL399043 THIOLACTOMYCIN IC50 = 1000.0 nM 6.00
CHEMBL1417019 MITOXANTRONE HYDROCHLORIDE IC50 = 2000.0 nM 5.70