Assay Detail
Binding
CHEMBL4185157
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of full length human APE1 expressed in Escherichia coli BL21/DE3 assessed as inhibition of incision of depurinated supercoiled pUC18 plasmid DNA after 15 mins
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
DNA repair nuclease/redox regulator APEX1 (CHEMBL5619) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Inhibitors of nuclease and redox activity of apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APE1/Ref-1).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 6.20 | 7.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL22077 | HYCANTHONE | 2.0 | 1 | 7.10 |
| CHEMBL279014 | LUCANTHONE | 2.0 | 1 | 5.30 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL22077 | HYCANTHONE | IC50 | = | 80.0 | nM | 7.10 |
| CHEMBL279014 | LUCANTHONE | IC50 | = | 5000.0 | nM | 5.30 |