Assay Detail
Binding
CHEMBL4185508
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of BRAF (unknown origin) using MEK1 K97R mutant as substrate preincubated for 60 to 120 mins followed by substrate addition measured after 2 hrs by TR-FRET assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Serine/threonine-protein kinase B-raf (CHEMBL5145) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Interrogating the Roles of Post-Translational Modifications of Non-Histone Proteins.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 7.64 | 7.64 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4209157 | LIFIRAFENIB | 2.0 | 1 | 7.64 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4209157 | LIFIRAFENIB | IC50 | = | 23.0 | nM | 7.64 |