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Assay Detail

CHEMBL4186847

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MLL1 binding to N-terminal His-tagged WRD5 23 deletion mutant (24 to 334 residues) (unknown origin) expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells by fluorescence polarization assay
6
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Histone-lysine N-methyltransferase 2A (CHEMBL1293299)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Targeting protein-protein interaction between MLL1 and reciprocal proteins for leukemia therapy.
Wang ZH, Li DD, Chen WL, You QD, Guo XK.
Bioorg Med Chem (2018) 26 : 356 -365
PubMed 29254892 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 8.50 8.62
Ki 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3883592 2 8.62
CHEMBL3799591 2 8.54
CHEMBL3957384 2 8.35

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3883592 IC50 = 2.4 nM 8.62
CHEMBL3799591 IC50 = 2.9 nM 8.54
CHEMBL3957384 IC50 = 4.5 nM 8.35
CHEMBL3799591 Ki < 1.0 nM -
CHEMBL3883592 Ki < 1.0 nM -
CHEMBL3957384 Ki < 1.0 nM -