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Assay Detail

CHEMBL4186929

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-ketanserin from human 5-HT2A receptor expressed in CHO-K1 cell membranes after 60 mins by microbeta scintillation counting analysis
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2A (CHEMBL224)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

5-HT1A and 5-HT2A receptors affinity, docking studies and pharmacological evaluation of a series of 8-acetyl-7-hydroxy-4-methylcoumarin derivatives.
Ostrowska K, Grzeszczuk D, Głuch-Lutwin M, Gryboś A, Siwek A, Leśniak A, Sacharczuk M, Trzaskowski B.
Bioorg Med Chem (2018) 26 : 527 -535
PubMed 29269256 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 7.60 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL6437 MIANSERIN 4.0 1 8.70
CHEMBL4215550 1 8.22
CHEMBL4214598 1 8.19
CHEMBL4218720 1 8.15
CHEMBL4215022 1 8.10
CHEMBL4207177 1 8.00
CHEMBL4213170 1 7.90
CHEMBL4217736 1 7.70
CHEMBL4207990 1 7.70
CHEMBL4209403 1 7.66
CHEMBL4212058 1 7.33
CHEMBL4207585 1 7.31
CHEMBL4218830 1 7.25
CHEMBL4202831 1 7.24
CHEMBL4213789 1 6.99
CHEMBL4212943 1 6.40
CHEMBL4210596 1 6.39

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL6437 MIANSERIN Ki = 2.0 nM 8.70
CHEMBL4215550 Ki = 6.0 nM 8.22
CHEMBL4214598 Ki = 6.5 nM 8.19
CHEMBL4218720 Ki = 7.0 nM 8.15
CHEMBL4215022 Ki = 8.0 nM 8.10
CHEMBL4207177 Ki = 10.0 nM 8.00
CHEMBL4213170 Ki = 12.5 nM 7.90
CHEMBL4217736 Ki = 20.0 nM 7.70
CHEMBL4207990 Ki = 20.0 nM 7.70
CHEMBL4209403 Ki = 22.0 nM 7.66
CHEMBL4212058 Ki = 46.8 nM 7.33
CHEMBL4207585 Ki = 48.7 nM 7.31
CHEMBL4218830 Ki = 56.0 nM 7.25
CHEMBL4202831 Ki = 58.0 nM 7.24
CHEMBL4213789 Ki = 102.0 nM 6.99
CHEMBL4212943 Ki = 400.0 nM 6.40
CHEMBL4210596 Ki = 408.0 nM 6.39