Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4187320

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant wild-type HIV-1 3B reverse transcriptase p66/p51 RNA-dependent DNA polymerase activity expressed in Escherichia coli JM109 assessed as reduction in dTTP incorporation using poly(rA)/oligo(dT)16 as template/primer after 40 mins by PicoGreen dye based spectrofluorometric analysis
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Human immunodeficiency virus 1
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Human immunodeficiency virus type 1 reverse transcriptase (CHEMBL247)
Type SINGLE PROTEIN
Organism Human immunodeficiency virus 1

Publication

Structural optimization of N1-aryl-benzimidazoles for the discovery of new non-nucleoside reverse transcriptase inhibitors active against wild-type and mutant HIV-1 strains.
Monforte AM, De Luca L, Buemi MR, Agharbaoui FE, Pannecouque C, Ferro S.
Bioorg Med Chem (2018) 26 : 661 -674
PubMed 29291935 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 5.86 7.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL223228 EFAVIRENZ 4.0 1 7.72
CHEMBL4218333 1 7.33
CHEMBL4203336 1 7.30
CHEMBL4211084 1 7.06
CHEMBL4211907 1 7.04
CHEMBL3114049 1 6.92
CHEMBL4207026 1 6.89
CHEMBL3114034 1 6.75
CHEMBL4202681 1 6.65
CHEMBL4218422 1 6.12
CHEMBL57 NEVIRAPINE 4.0 1 5.81
CHEMBL4210982 1 5.26
CHEMBL4204242 1 5.23
CHEMBL4211390 1 5.12
CHEMBL4213853 1 4.80
CHEMBL4214858 1 4.80
CHEMBL3114032 1 4.73
CHEMBL4203211 1 4.67
CHEMBL4205972 1 4.33
CHEMBL3114057 1 4.29
CHEMBL4216358 1 4.22
CHEMBL3114045 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL223228 EFAVIRENZ IC50 = 19.0 nM 7.72
CHEMBL4218333 IC50 = 47.0 nM 7.33
CHEMBL4203336 IC50 = 50.0 nM 7.30
CHEMBL4211084 IC50 = 88.0 nM 7.06
CHEMBL4211907 IC50 = 91.0 nM 7.04
CHEMBL3114049 IC50 = 120.0 nM 6.92
CHEMBL4207026 IC50 = 130.0 nM 6.89
CHEMBL3114034 IC50 = 180.0 nM 6.75
CHEMBL4202681 IC50 = 222.0 nM 6.65
CHEMBL4218422 IC50 = 760.0 nM 6.12
CHEMBL57 NEVIRAPINE IC50 = 1550.0 nM 5.81
CHEMBL4210982 IC50 = 5450.0 nM 5.26
CHEMBL4204242 IC50 = 5840.0 nM 5.23
CHEMBL4211390 IC50 = 7680.0 nM 5.12
CHEMBL4213853 IC50 = 15740.0 nM 4.80
CHEMBL4214858 IC50 = 15670.0 nM 4.80
CHEMBL3114032 IC50 = 18510.0 nM 4.73
CHEMBL4203211 IC50 = 21340.0 nM 4.67
CHEMBL4205972 IC50 = 47300.0 nM 4.33
CHEMBL3114057 IC50 = 51930.0 nM 4.29
CHEMBL4216358 IC50 = 60610.0 nM 4.22
CHEMBL3114045 IC50 = 162170.0 nM -