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Assay Detail

CHEMBL4187777

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human DPP4 using A-P-7-amido-4-trifluoromethylcoumarin as substrate pretreated for 10 mins followed by substrate addition measured after 10 mins
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dipeptidyl peptidase 4 (CHEMBL284)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Surrogating and redirection of pyrazolo[1,5-a]pyrimidin-7(4H)-one core, a novel class of potent and selective DPP-4 inhibitors.
Deng X, Shen J, Zhu H, Xiao J, Sun R, Xie F, Lam C, Wang J, Qiao Y, Tavallaie MS, Hu Y, Du Y, Li J, Fu L, Jiang F.
Bioorg Med Chem (2018) 26 : 903 -912
PubMed 29373269 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.73 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL376359 ALOGLIPTIN 4.0 1 8.40
CHEMBL4205581 1 7.31
CHEMBL4204176 1 7.10
CHEMBL4212894 1 6.40
CHEMBL4213950 1 5.92
CHEMBL4215849 1 5.89
CHEMBL4207996 1 5.70
CHEMBL4208513 1 5.60
CHEMBL4216792 1 4.98
CHEMBL4212367 1 4.94
CHEMBL4217326 1 4.76
CHEMBL4210635 1 4.67
CHEMBL4203639 1 4.33
CHEMBL4205100 1 4.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL376359 ALOGLIPTIN IC50 = 4.0 nM 8.40
CHEMBL4205581 IC50 = 49.0 nM 7.31
CHEMBL4204176 IC50 = 80.0 nM 7.10
CHEMBL4212894 IC50 = 400.0 nM 6.40
CHEMBL4213950 IC50 = 1200.0 nM 5.92
CHEMBL4215849 IC50 = 1300.0 nM 5.89
CHEMBL4207996 IC50 = 2000.0 nM 5.70
CHEMBL4208513 IC50 = 2500.0 nM 5.60
CHEMBL4216792 IC50 = 10400.0 nM 4.98
CHEMBL4212367 IC50 = 11500.0 nM 4.94
CHEMBL4217326 IC50 = 17200.0 nM 4.76
CHEMBL4210635 IC50 = 21500.0 nM 4.67
CHEMBL4203639 IC50 = 46500.0 nM 4.33
CHEMBL4205100 IC50 = 59900.0 nM 4.22