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Assay Detail

CHEMBL4188423

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NS5B polymerase in HCV genotype 1b infected human HuH7 replicon cells assessed as reduction in viral RNA level after 3 days by RT-PCR assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus subtype 1b
Confidence 8 — Homologous single protein target
Curated By Intermediate

Target

RNA-directed RNA polymerase (CHEMBL4296320)
Type SINGLE PROTEIN
Organism Hepacivirus hominis

Publication

Discovery of novel potent HCV NS5B polymerase non-nucleoside inhibitors bearing a fused benzofuran scaffold.
Zhong M, Peng E, Huang N, Huang Q, Huq A, Lau M, Colonno R, Li L.
Bioorg Med Chem Lett (2018) 28 : 963 -968
PubMed 29422387 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 1 8.05 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1092581 NESBUVIR 2.0 1 8.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1092581 NESBUVIR EC50 = 9.0 nM 8.05