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Assay Detail

CHEMBL4188426

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HCV genotype 2a NS5B polymerase infected in human HuH7 replicon cells by luciferase reporter gene assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus subtype 2a
Confidence 8 — Homologous single protein target
Curated By Intermediate

Target

RNA-directed RNA polymerase (CHEMBL4296320)
Type SINGLE PROTEIN
Organism Hepacivirus hominis

Publication

Discovery of novel potent HCV NS5B polymerase non-nucleoside inhibitors bearing a fused benzofuran scaffold.
Zhong M, Peng E, Huang N, Huang Q, Huq A, Lau M, Colonno R, Li L.
Bioorg Med Chem Lett (2018) 28 : 963 -968
PubMed 29422387 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 7 7.28 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4204025 1 7.92
CHEMBL4214242 1 7.75
CHEMBL4208902 1 7.21
CHEMBL4211784 1 7.19
CHEMBL1092581 NESBUVIR 2.0 1 7.14
CHEMBL4206056 1 6.87
CHEMBL4211447 1 6.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4204025 EC50 = 12.0 nM 7.92
CHEMBL4214242 EC50 = 18.0 nM 7.75
CHEMBL4208902 EC50 = 62.0 nM 7.21
CHEMBL4211784 EC50 = 64.0 nM 7.19
CHEMBL1092581 NESBUVIR EC50 = 72.0 nM 7.14
CHEMBL4206056 EC50 = 134.0 nM 6.87
CHEMBL4211447 EC50 = 141.0 nM 6.85