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Assay Detail

CHEMBL4188522

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human full length CDK6 expressed in baculovirus infected Sf9 insect cells using histone H1 substrate after 90 mins by ADP-Glo assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cyclin-dependent kinase 6 (CHEMBL2508)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of 4-(2,3-dihydro-1H-benzo[d]pyrrolo[1,2-a]imidazol-7-yl)-N-(5-(piperazin-1-ylmethyl)pyridine-2-yl)pyrimidin-2-amine as a highly potent and selective cyclin-dependent kinases 4 and 6 inhibitors and the discovery of structure-activity relationships.
Wang Y, Liu WJ, Yin L, Li H, Chen ZH, Zhu DX, Song XQ, Cheng ZZ, Song P, Wang Z, Li ZG.
Bioorg Med Chem Lett (2018) 28 : 974 -978
PubMed 29429832 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 7.45 9.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4208172 1 9.05
CHEMBL4217694 1 8.62
CHEMBL4209329 1 8.43
CHEMBL4215702 1 8.40
CHEMBL3301610 ABEMACICLIB 4.0 1 8.11
CHEMBL4210707 1 7.95
CHEMBL4211828 1 7.90
CHEMBL4210028 1 7.80
CHEMBL4205645 1 7.76
CHEMBL4207474 1 7.73
CHEMBL4207451 1 7.72
CHEMBL4213847 1 7.66
CHEMBL4212542 1 7.55
CHEMBL4207804 1 7.44
CHEMBL4211479 1 7.34
CHEMBL4208366 1 7.34
CHEMBL4215126 1 6.75
CHEMBL4204961 1 6.61
CHEMBL4214566 1 6.61
CHEMBL4216447 1 6.57
CHEMBL4209318 1 6.56
CHEMBL4213109 1 6.47
CHEMBL4215568 1 6.41
CHEMBL4207966 1 6.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4208172 IC50 = 0.9 nM 9.05
CHEMBL4217694 IC50 = 2.4 nM 8.62
CHEMBL4209329 IC50 = 3.7 nM 8.43
CHEMBL4215702 IC50 = 4.0 nM 8.40
CHEMBL3301610 ABEMACICLIB IC50 = 7.8 nM 8.11
CHEMBL4210707 IC50 = 11.2 nM 7.95
CHEMBL4211828 IC50 = 12.7 nM 7.90
CHEMBL4210028 IC50 = 15.8 nM 7.80
CHEMBL4205645 IC50 = 17.4 nM 7.76
CHEMBL4207474 IC50 = 18.7 nM 7.73
CHEMBL4207451 IC50 = 19.0 nM 7.72
CHEMBL4213847 IC50 = 22.0 nM 7.66
CHEMBL4212542 IC50 = 28.0 nM 7.55
CHEMBL4207804 IC50 = 36.0 nM 7.44
CHEMBL4211479 IC50 = 46.0 nM 7.34
CHEMBL4208366 IC50 = 45.6 nM 7.34
CHEMBL4215126 IC50 = 178.0 nM 6.75
CHEMBL4204961 IC50 = 247.8 nM 6.61
CHEMBL4214566 IC50 = 244.1 nM 6.61
CHEMBL4216447 IC50 = 271.0 nM 6.57
CHEMBL4209318 IC50 = 275.0 nM 6.56
CHEMBL4213109 IC50 = 339.0 nM 6.47
CHEMBL4215568 IC50 = 392.0 nM 6.41
CHEMBL4207966 IC50 = 752.0 nM 6.12