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Assay Detail

CHEMBL4189462

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]PSB-603 from recombinant mouse adenosine A2B receptor expressed in CHO cell membranes after 75 mins by liquid scintillation counting method
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2b (CHEMBL2237)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Fluorescent-Labeled Selective Adenosine A2B Receptor Antagonist Enables Competition Binding Assay by Flow Cytometry.
Köse M, Gollos S, Karcz T, Fiene A, Heisig F, Behrenswerth A, Kieć-Kononowicz K, Namasivayam V, Müller CE.
J Med Chem (2018) 61 : 4301 -4316
PubMed 29681156 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 6.76 8.88

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4205635 1 8.88
CHEMBL113 CAFFEINE 4.0 1 4.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4205635 Ki = 1.32 nM 8.88
CHEMBL113 CAFFEINE Ki = 23000.0 nM 4.64