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Assay Detail

CHEMBL4189497

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-tagged MNK2 (72 to 385 residues) (unknown origin) expressed in Escherichia coli BL21(DE3) using JH3 peptide as substrate preincubated for 10 mins followed by substrate and ATP addition measured after 60 mins
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Optimization of Selective Mitogen-Activated Protein Kinase Interacting Kinases 1 and 2 Inhibitors for the Treatment of Blast Crisis Leukemia.
Yang H, Chennamaneni LR, Ho MWT, Ang SH, Tan ESW, Jeyaraj DA, Yeap YS, Liu B, Ong EH, Joy JK, Wee JLK, Kwek P, Retna P, Dinie N, Nguyen TTH, Tai SJ, Manoharan V, Pendharkar V, Low CB, Chew YS, Vuddagiri S, Sangthongpitag K, Choong ML, Lee MA, Kannan S, Verma CS, Poulsen A, Lim S, Chuah C, Ong TS, Hill J, Matter A, Nacro K.
J Med Chem (2018) 61 : 4348 -4369
PubMed 29683667 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 6.69 7.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4216109 1 7.96
CHEMBL4213168 1 7.60
CHEMBL4208772 1 7.58
CHEMBL4214092 1 7.51
CHEMBL4203889 1 7.39
CHEMBL4204438 1 7.14
CHEMBL4211649 TINODASERTIB 2.0 1 7.07
CHEMBL4209905 1 6.95
CHEMBL4209496 1 6.85
CHEMBL4208246 1 6.84
CHEMBL4208890 1 6.80
CHEMBL4204506 1 6.73
CHEMBL4212480 1 6.71
CHEMBL4211757 1 6.66
CHEMBL4202593 1 6.47
CHEMBL4217366 1 6.45
CHEMBL4212200 1 6.41
CHEMBL4212307 1 6.39
CHEMBL4213023 1 6.38
CHEMBL4213819 1 6.34
CHEMBL4216991 1 6.30
CHEMBL4208627 1 6.29
CHEMBL4216093 1 6.13
CHEMBL4208112 1 5.97
CHEMBL4206349 1 5.84
CHEMBL4202639 1 5.29

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4216109 IC50 = 11.0 nM 7.96
CHEMBL4213168 IC50 = 25.0 nM 7.60
CHEMBL4208772 IC50 = 26.0 nM 7.58
CHEMBL4214092 IC50 = 31.0 nM 7.51
CHEMBL4203889 IC50 = 41.0 nM 7.39
CHEMBL4204438 IC50 = 72.0 nM 7.14
CHEMBL4211649 TINODASERTIB IC50 = 86.0 nM 7.07
CHEMBL4209905 IC50 = 113.0 nM 6.95
CHEMBL4209496 IC50 = 140.0 nM 6.85
CHEMBL4208246 IC50 = 143.0 nM 6.84
CHEMBL4208890 IC50 = 160.0 nM 6.80
CHEMBL4204506 IC50 = 187.0 nM 6.73
CHEMBL4212480 IC50 = 193.0 nM 6.71
CHEMBL4211757 IC50 = 217.0 nM 6.66
CHEMBL4202593 IC50 = 336.0 nM 6.47
CHEMBL4217366 IC50 = 355.0 nM 6.45
CHEMBL4212200 IC50 = 389.0 nM 6.41
CHEMBL4212307 IC50 = 410.0 nM 6.39
CHEMBL4213023 IC50 = 415.0 nM 6.38
CHEMBL4213819 IC50 = 454.0 nM 6.34
CHEMBL4216991 IC50 = 502.0 nM 6.30
CHEMBL4208627 IC50 = 515.0 nM 6.29
CHEMBL4216093 IC50 = 742.0 nM 6.13
CHEMBL4208112 IC50 = 1080.0 nM 5.97
CHEMBL4206349 IC50 = 1430.0 nM 5.84
CHEMBL4202639 IC50 = 5170.0 nM 5.29