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Assay Detail

CHEMBL4189498

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Binding
Inhibition of MNK1/2 in human HeLa cells assessed as decrease in eIF4E phosphorylation at Ser209 after 2 hrs by AlphaScreen assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 5 — Multiple protein complex / RNA
Curated By Intermediate

Publication

Optimization of Selective Mitogen-Activated Protein Kinase Interacting Kinases 1 and 2 Inhibitors for the Treatment of Blast Crisis Leukemia.
Yang H, Chennamaneni LR, Ho MWT, Ang SH, Tan ESW, Jeyaraj DA, Yeap YS, Liu B, Ong EH, Joy JK, Wee JLK, Kwek P, Retna P, Dinie N, Nguyen TTH, Tai SJ, Manoharan V, Pendharkar V, Low CB, Chew YS, Vuddagiri S, Sangthongpitag K, Choong ML, Lee MA, Kannan S, Verma CS, Poulsen A, Lim S, Chuah C, Ong TS, Hill J, Matter A, Nacro K.
J Med Chem (2018) 61 : 4348 -4369
PubMed 29683667 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 6.24 6.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4209905 1 6.89
CHEMBL4209496 1 6.72
CHEMBL4208772 1 6.66
CHEMBL4204438 1 6.64
CHEMBL4213168 1 6.49
CHEMBL4211649 TINODASERTIB 2.0 1 6.49
CHEMBL4203889 1 6.38
CHEMBL4216109 1 6.37
CHEMBL4216991 1 6.36
CHEMBL4208246 1 6.19
CHEMBL4213819 1 6.13
CHEMBL4211757 1 6.12
CHEMBL4213023 1 6.09
CHEMBL4212480 1 5.97
CHEMBL4212307 1 5.92
CHEMBL4216093 1 5.88
CHEMBL4202593 1 5.79
CHEMBL4206349 1 5.72
CHEMBL4212200 1 5.70
CHEMBL4217366 1 -
CHEMBL4214092 1 -
CHEMBL4204506 1 -
CHEMBL4208890 1 -
CHEMBL4208627 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4209905 IC50 = 130.0 nM 6.89
CHEMBL4209496 IC50 = 189.0 nM 6.72
CHEMBL4208772 IC50 = 220.0 nM 6.66
CHEMBL4204438 IC50 = 229.0 nM 6.64
CHEMBL4213168 IC50 = 326.0 nM 6.49
CHEMBL4211649 TINODASERTIB IC50 = 321.0 nM 6.49
CHEMBL4203889 IC50 = 421.0 nM 6.38
CHEMBL4216109 IC50 = 431.0 nM 6.37
CHEMBL4216991 IC50 = 440.0 nM 6.36
CHEMBL4208246 IC50 = 640.0 nM 6.19
CHEMBL4213819 IC50 = 748.0 nM 6.13
CHEMBL4211757 IC50 = 759.0 nM 6.12
CHEMBL4213023 IC50 = 819.0 nM 6.09
CHEMBL4212480 IC50 = 1080.0 nM 5.97
CHEMBL4212307 IC50 = 1210.0 nM 5.92
CHEMBL4216093 IC50 = 1330.0 nM 5.88
CHEMBL4202593 IC50 = 1630.0 nM 5.79
CHEMBL4206349 IC50 = 1920.0 nM 5.72
CHEMBL4212200 IC50 = 1990.0 nM 5.70
CHEMBL4217366 IC50 > 2500.0 nM -
CHEMBL4214092 IC50 > 2500.0 nM -
CHEMBL4204506 IC50 > 2500.0 nM -
CHEMBL4208890 IC50 > 2500.0 nM -
CHEMBL4208627 IC50 > 2500.0 nM -