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Assay Detail

CHEMBL4190120

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ERK1/2 in human A375 cells harboring BRAF V600E mutant assessed as suppression of RSK phosphorylation after 4 hrs by MSD assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Intermediate

Target

Mitogen-activated protein kinase; ERK1/ERK2 (CHEMBL1907606)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Fragment-Based Discovery of a Potent, Orally Bioavailable Inhibitor That Modulates the Phosphorylation and Catalytic Activity of ERK1/2.
Heightman TD, Berdini V, Braithwaite H, Buck IM, Cassidy M, Castro J, Courtin A, Day JEH, East C, Fazal L, Graham B, Griffiths-Jones CM, Lyons JF, Martins V, Muench S, Munck JM, Norton D, O'Reilly M, Palmer N, Pathuri P, Reader M, Rees DC, Rich SJ, Richardson C, Saini H, Thompson NT, Wallis NG, Walton H, Wilsher NE, Woolford AJ, Cooke M, Cousin D, Onions S, Shannon J, Watts J, Murray CW.
J Med Chem (2018) 61 : 4978 -4992
PubMed 29775310 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.33 8.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4212211 1 8.46
CHEMBL4210682 1 6.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4212211 IC50 = 3.5 nM 8.46
CHEMBL4210682 IC50 = 630.0 nM 6.20