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Assay Detail

CHEMBL4190137

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of C-terminal His-tagged recombinant human CYP21A2deltaH mutant expressed in Escherichia coli DH5alpha assessed as decrease in progesterone hydroxylation preincubated for 3 mins followed by NADPH addition measured after 10 mins in presence of cytochrome p450 reductase by LC-MS/MS method
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Steroid 21-hydroxylase (CHEMBL2759)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-Based Design of Inhibitors with Improved Selectivity for Steroidogenic Cytochrome P450 17A1 over Cytochrome P450 21A2.
Fehl C, Vogt CD, Yadav R, Li K, Scott EE, Aubé J.
J Med Chem (2018) 61 : 4946 -4960
PubMed 29792703 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.92 9.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL132778 1 9.15
CHEMBL4209941 1 7.62
CHEMBL254328 ABIRATERONE 3.0 1 7.49
CHEMBL4210737 1 7.38
CHEMBL2105738 GALETERONE 3.0 1 7.11
CHEMBL4213452 1 6.63
CHEMBL4209916 1 6.36
CHEMBL4210141 1 6.17
CHEMBL4214976 1 5.86
CHEMBL4210952 1 5.44
CHEMBL4203137 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL132778 IC50 = 0.7 nM 9.15
CHEMBL4209941 IC50 = 23.9 nM 7.62
CHEMBL254328 ABIRATERONE IC50 = 32.4 nM 7.49
CHEMBL4210737 IC50 = 42.1 nM 7.38
CHEMBL2105738 GALETERONE IC50 = 77.2 nM 7.11
CHEMBL4213452 IC50 = 234.0 nM 6.63
CHEMBL4209916 IC50 = 440.0 nM 6.36
CHEMBL4210141 IC50 = 673.0 nM 6.17
CHEMBL4214976 IC50 = 1390.0 nM 5.86
CHEMBL4210952 IC50 = 3605.0 nM 5.44
CHEMBL4203137 IC50 >= 700.0 nM -