Assay Detail
Binding
CHEMBL4190587
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Inhibition of human recombinant SIRT1 using AMC-p53 (379 to 382 residues) as substrate incubated for 45 mins followed by NAD+ addition measured after 30 mins by fluorescence assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
NAD-dependent protein deacetylase sirtuin-1 (CHEMBL4506) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 12 | 4.86 | 6.52 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL420311 | — | — | 1 | 6.52 |
| CHEMBL4216715 | — | — | 1 | 4.99 |
| CHEMBL4202895 | — | — | 1 | 4.70 |
| CHEMBL4210076 | — | — | 1 | 4.53 |
| CHEMBL4212277 | — | — | 1 | 4.46 |
| CHEMBL4214892 | — | — | 1 | 4.43 |
| CHEMBL595354 | — | — | 1 | 4.38 |
| CHEMBL4217034 | — | — | 1 | - |
| CHEMBL224864 | — | — | 1 | - |
| CHEMBL4211748 | — | — | 1 | - |
| CHEMBL4218670 | — | — | 1 | - |
| CHEMBL4218205 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL420311 | — | IC50 | = | 300.0 | nM | 6.52 |
| CHEMBL4216715 | — | IC50 | = | 10220.0 | nM | 4.99 |
| CHEMBL4202895 | — | IC50 | = | 19960.0 | nM | 4.70 |
| CHEMBL4210076 | — | IC50 | = | 29800.0 | nM | 4.53 |
| CHEMBL4212277 | — | IC50 | = | 35070.0 | nM | 4.46 |
| CHEMBL4214892 | — | IC50 | = | 36970.0 | nM | 4.43 |
| CHEMBL595354 | — | IC50 | = | 42100.0 | nM | 4.38 |
| CHEMBL4217034 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL224864 | — | IC50 | - | — | - | |
| CHEMBL4211748 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL4218670 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL4218205 | — | IC50 | > | 50000.0 | nM | - |