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Assay Detail

CHEMBL4190835

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant FLAG-tagged full length ATR expressed in mammalian cell line co-expressing human recombinant cMyc-tagged full length ATRIP
24
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase ATR (CHEMBL5024)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of pyrazolopyrimidine derivatives as novel inhibitors of ataxia telangiectasia and rad3 related protein (ATR).
Ramachandran SA, Jadhavar PS, Singh MP, Sharma A, Bagle GN, Quinn KP, Wong PY, Protter AA, Rai R, Pham SM, Lindquist JN.
Bioorg Med Chem Lett (2017) 27 : 750 -754
PubMed 28131712 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 6.05 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3989870 BERZOSERTIB 2.0 1 7.82
CHEMBL4207014 1 7.18
CHEMBL4211377 1 7.07
CHEMBL4207265 3 6.80
CHEMBL4207205 2 6.57
CHEMBL4213994 1 6.24
CHEMBL4210861 1 6.18
CHEMBL4215278 1 5.88
CHEMBL1081312 1 5.85
CHEMBL4209086 1 5.80
CHEMBL4211185 1 5.73
CHEMBL4216036 1 5.58
CHEMBL3964073 1 5.45
CHEMBL4210228 1 5.33
CHEMBL4215814 1 4.72
CHEMBL4204301 1 4.71
CHEMBL4209026 1 4.65
CHEMBL4212315 1 -
CHEMBL4203385 1 -
CHEMBL4212747 1 -
CHEMBL4206529 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3989870 BERZOSERTIB IC50 = 15.0 nM 7.82
CHEMBL4207014 IC50 = 66.0 nM 7.18
CHEMBL4211377 IC50 = 86.0 nM 7.07
CHEMBL4207265 IC50 = 158.0 nM 6.80
CHEMBL4207265 IC50 = 212.0 nM 6.67
CHEMBL4207205 IC50 = 269.0 nM 6.57
CHEMBL4207265 IC50 = 304.0 nM 6.52
CHEMBL4207205 IC50 = 547.0 nM 6.26
CHEMBL4213994 IC50 = 580.0 nM 6.24
CHEMBL4210861 IC50 = 654.0 nM 6.18
CHEMBL4215278 IC50 = 1320.0 nM 5.88
CHEMBL1081312 IC50 = 1420.0 nM 5.85
CHEMBL4209086 IC50 = 1590.0 nM 5.80
CHEMBL4211185 IC50 = 1860.0 nM 5.73
CHEMBL4216036 IC50 = 2630.0 nM 5.58
CHEMBL3964073 IC50 = 3560.0 nM 5.45
CHEMBL4210228 IC50 = 4620.0 nM 5.33
CHEMBL4215814 IC50 = 19100.0 nM 4.72
CHEMBL4204301 IC50 = 19300.0 nM 4.71
CHEMBL4209026 IC50 = 22400.0 nM 4.65
CHEMBL4212747 IC50 > 30000.0 nM -
CHEMBL4212315 IC50 > 30000.0 nM -
CHEMBL4203385 IC50 > 30000.0 nM -
CHEMBL4206529 IC50 > 30000.0 nM -