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Assay Detail

CHEMBL4191650

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of wild type GST-tagged RGS17 (unknown origin) interaction with biotinylated Galphao after 30 mins in presence of AMF and GDP by AlphaScreen assay
6
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Regulator of G-protein signaling 17 (CHEMBL4295974)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Natural Products Discovered in a High-Throughput Screen Identified as Inhibitors of RGS17 and as Cytostatic and Cytotoxic Agents for Lung and Prostate Cancer Cell Lines.
Bodle CR, Mackie DI, Hayes MP, Schamp JH, Miller MR, Henry MD, Doorn JA, Houtman JCD, James MA, Roman DL.
J Nat Prod (2017) 80 : 1992 -2000
PubMed 28621943 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.48 4.98
Inhibition 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL301982 CELASTROL 2 4.98
CHEMBL1438439 IRIGENOL 2 4.33
CHEMBL417799 SANGUINARIUM 2.0 2 4.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL301982 CELASTROL IC50 = 10500.0 nM 4.98
CHEMBL1438439 IRIGENOL IC50 = 47200.0 nM 4.33
CHEMBL417799 SANGUINARIUM IC50 = 72800.0 nM 4.14
CHEMBL417799 SANGUINARIUM Inhibition - % -
CHEMBL301982 CELASTROL Inhibition - % -
CHEMBL1438439 IRIGENOL Inhibition - % -