Assay Detail
Functional
CHEMBL4194087
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability using compound addition to cell culture cells in log phase of growth and incubated for 48 hrs by MTT assay
65
Total Activities
65
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | MCF7 |
| Confidence | 1 — Organism |
| Curated By | Intermediate |
Publication
Structure-based design, synthesis, and biological evaluation of withaferin A-analogues as potent apoptotic inducers.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 65 | 5.56 | 7.32 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1554 | DACTINOMYCIN | 4.0 | 1 | 7.32 |
| CHEMBL4216031 | — | — | 1 | 6.40 |
| CHEMBL4204433 | — | — | 1 | 6.22 |
| CHEMBL4206549 | — | — | 1 | 6.16 |
| CHEMBL3234472 | — | — | 1 | 6.10 |
| CHEMBL4209797 | — | — | 1 | 6.05 |
| CHEMBL4209302 | — | — | 1 | 6.00 |
| CHEMBL4208173 | — | — | 1 | 6.00 |
| CHEMBL4203302 | — | — | 1 | 6.00 |
| CHEMBL4218090 | — | — | 1 | 6.00 |
| CHEMBL4204260 | — | — | 1 | 6.00 |
| CHEMBL4212265 | — | — | 1 | 5.96 |
| CHEMBL4215548 | — | — | 1 | 5.96 |
| CHEMBL4206340 | — | — | 1 | 5.92 |
| CHEMBL4207375 | — | — | 1 | 5.92 |
| CHEMBL2047418 | — | — | 1 | 5.92 |
| CHEMBL4211069 | — | — | 1 | 5.92 |
| CHEMBL517080 | WITHAFERIN A | 1.0 | 1 | 5.89 |
| CHEMBL4207645 | — | — | 1 | 5.82 |
| CHEMBL4212549 | — | — | 1 | 5.80 |
| CHEMBL4214591 | — | — | 1 | 5.77 |
| CHEMBL4217570 | — | — | 1 | 5.77 |
| CHEMBL4206786 | — | — | 1 | 5.72 |
| CHEMBL4210205 | — | — | 1 | 5.68 |
| CHEMBL4208766 | — | — | 1 | 5.66 |
| CHEMBL4213162 | — | — | 1 | 5.64 |
| CHEMBL4210710 | — | — | 1 | 5.64 |
| CHEMBL4202923 | — | — | 1 | 5.62 |
| CHEMBL4218054 | — | — | 1 | 5.55 |
| CHEMBL4209365 | — | — | 1 | 5.51 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1554 | DACTINOMYCIN | IC50 | = | 48.0 | nM | 7.32 |
| CHEMBL4216031 | — | IC50 | = | 400.0 | nM | 6.40 |
| CHEMBL4204433 | — | IC50 | = | 600.0 | nM | 6.22 |
| CHEMBL4206549 | — | IC50 | = | 700.0 | nM | 6.16 |
| CHEMBL3234472 | — | IC50 | = | 800.0 | nM | 6.10 |
| CHEMBL4209797 | — | IC50 | = | 900.0 | nM | 6.05 |
| CHEMBL4209302 | — | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL4208173 | — | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL4203302 | — | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL4218090 | — | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL4204260 | — | IC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL4212265 | — | IC50 | = | 1100.0 | nM | 5.96 |
| CHEMBL4215548 | — | IC50 | = | 1100.0 | nM | 5.96 |
| CHEMBL2047418 | — | IC50 | = | 1200.0 | nM | 5.92 |
| CHEMBL4207375 | — | IC50 | = | 1200.0 | nM | 5.92 |
| CHEMBL4206340 | — | IC50 | = | 1200.0 | nM | 5.92 |
| CHEMBL4211069 | — | IC50 | = | 1200.0 | nM | 5.92 |
| CHEMBL517080 | WITHAFERIN A | IC50 | = | 1300.0 | nM | 5.89 |
| CHEMBL4207645 | — | IC50 | = | 1500.0 | nM | 5.82 |
| CHEMBL4212549 | — | IC50 | = | 1600.0 | nM | 5.80 |
| CHEMBL4214591 | — | IC50 | = | 1700.0 | nM | 5.77 |
| CHEMBL4217570 | — | IC50 | = | 1700.0 | nM | 5.77 |
| CHEMBL4206786 | — | IC50 | = | 1900.0 | nM | 5.72 |
| CHEMBL4210205 | — | IC50 | = | 2100.0 | nM | 5.68 |
| CHEMBL4208766 | — | IC50 | = | 2200.0 | nM | 5.66 |
| CHEMBL4213162 | — | IC50 | = | 2300.0 | nM | 5.64 |
| CHEMBL4210710 | — | IC50 | = | 2300.0 | nM | 5.64 |
| CHEMBL4202923 | — | IC50 | = | 2400.0 | nM | 5.62 |
| CHEMBL4218054 | — | IC50 | = | 2800.0 | nM | 5.55 |
| CHEMBL4209365 | — | IC50 | = | 3100.0 | nM | 5.51 |
| CHEMBL4215486 | — | IC50 | = | 3500.0 | nM | 5.46 |
| CHEMBL4208826 | — | IC50 | = | 4500.0 | nM | 5.35 |
| CHEMBL2047407 | — | IC50 | = | 4600.0 | nM | 5.34 |
| CHEMBL4217218 | — | IC50 | = | 4800.0 | nM | 5.32 |
| CHEMBL4218530 | — | IC50 | = | 4800.0 | nM | 5.32 |
| CHEMBL4215778 | — | IC50 | = | 5600.0 | nM | 5.25 |
| CHEMBL1425 | MERCAPTOPURINE ANHYDROUS | IC50 | = | 5800.0 | nM | 5.24 |
| CHEMBL4203569 | — | IC50 | = | 6600.0 | nM | 5.18 |
| CHEMBL3234486 | — | IC50 | = | 6800.0 | nM | 5.17 |
| CHEMBL4213754 | — | IC50 | = | 6800.0 | nM | 5.17 |
| CHEMBL4215247 | — | IC50 | = | 6900.0 | nM | 5.16 |
| CHEMBL4202626 | — | IC50 | = | 7200.0 | nM | 5.14 |
| CHEMBL4213674 | — | IC50 | = | 7500.0 | nM | 5.12 |
| CHEMBL2047417 | — | IC50 | = | 7900.0 | nM | 5.10 |
| CHEMBL4215133 | — | IC50 | = | 11000.0 | nM | 4.96 |
| CHEMBL1934585 | — | IC50 | = | 12200.0 | nM | 4.91 |
| CHEMBL4204997 | — | IC50 | = | 13800.0 | nM | 4.86 |
| CHEMBL4203046 | — | IC50 | = | 18500.0 | nM | 4.73 |
| CHEMBL4215138 | — | IC50 | = | 21600.0 | nM | 4.67 |
| CHEMBL4210211 | — | IC50 | = | 23000.0 | nM | 4.64 |