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Assay Detail

CHEMBL4195692

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against human HT-29 cells harboring BRAF V600E mutant after 24 hrs by MTT assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Intermediate

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

Design, synthesis, and biological evaluation of pyrazole derivatives containing acetamide bond as potential BRAFV600E inhibitors.
Wang CR, Wang ZF, Shi L, Wang ZC, Zhu HL.
Bioorg Med Chem Lett (2018) 28 : 2382 -2390
PubMed 29934244 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 4.90 5.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4209555 1 5.82
CHEMBL4206547 1 5.66
CHEMBL4211435 1 5.24
CHEMBL4210074 1 5.21
CHEMBL4217956 1 5.10
CHEMBL4210104 1 5.07
CHEMBL4213568 1 5.06
CHEMBL4208630 1 5.03
CHEMBL4215335 1 4.99
CHEMBL4205709 1 4.97
CHEMBL4205180 1 4.95
CHEMBL4209989 1 4.94
CHEMBL4218392 1 4.91
CHEMBL4217416 1 4.90
CHEMBL4203663 1 4.89
CHEMBL4210916 1 4.89
CHEMBL4215872 1 4.65
CHEMBL4203142 1 4.62
CHEMBL4208969 1 4.60
CHEMBL4214559 1 4.59
CHEMBL4208019 1 4.58
CHEMBL4204094 1 4.57
CHEMBL4205738 1 4.48
CHEMBL4213026 1 4.46
CHEMBL4211407 1 4.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4209555 IC50 = 1520.0 nM 5.82
CHEMBL4206547 IC50 = 2170.0 nM 5.66
CHEMBL4211435 IC50 = 5700.0 nM 5.24
CHEMBL4210074 IC50 = 6160.0 nM 5.21
CHEMBL4217956 IC50 = 7980.0 nM 5.10
CHEMBL4210104 IC50 = 8430.0 nM 5.07
CHEMBL4213568 IC50 = 8730.0 nM 5.06
CHEMBL4208630 IC50 = 9330.0 nM 5.03
CHEMBL4215335 IC50 = 10130.0 nM 4.99
CHEMBL4205709 IC50 = 10820.0 nM 4.97
CHEMBL4205180 IC50 = 11120.0 nM 4.95
CHEMBL4209989 IC50 = 11540.0 nM 4.94
CHEMBL4218392 IC50 = 12350.0 nM 4.91
CHEMBL4217416 IC50 = 12650.0 nM 4.90
CHEMBL4203663 IC50 = 12740.0 nM 4.89
CHEMBL4210916 IC50 = 12920.0 nM 4.89
CHEMBL4215872 IC50 = 22560.0 nM 4.65
CHEMBL4203142 IC50 = 24140.0 nM 4.62
CHEMBL4208969 IC50 = 25360.0 nM 4.60
CHEMBL4214559 IC50 = 25620.0 nM 4.59
CHEMBL4208019 IC50 = 26550.0 nM 4.58
CHEMBL4204094 IC50 = 26790.0 nM 4.57
CHEMBL4205738 IC50 = 32800.0 nM 4.48
CHEMBL4213026 IC50 = 35100.0 nM 4.46
CHEMBL4211407 IC50 = 49870.0 nM 4.30