Assay Detail
Binding
CHEMBL4199302
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human MIF expressed in Escherichia coli using HPP as substrate preincubated for 30 mins followed by substrate addition
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Macrophage migration inhibitory factor (CHEMBL2085) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Advances and Insights for Small Molecule Inhibition of Macrophage Migration Inhibitory Factor.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 4 | 6.28 | 7.25 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4208992 | — | — | 1 | 7.25 |
| CHEMBL3917143 | — | — | 1 | 7.24 |
| CHEMBL3798149 | — | — | 1 | 6.19 |
| CHEMBL1288318 | — | — | 1 | 4.43 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4208992 | — | Ki | = | 56.0 | nM | 7.25 |
| CHEMBL3917143 | — | Ki | = | 57.0 | nM | 7.24 |
| CHEMBL3798149 | — | Ki | = | 650.0 | nM | 6.19 |
| CHEMBL1288318 | — | Ki | = | 37000.0 | nM | 4.43 |