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Assay Detail

CHEMBL4199304

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human MIF tautomerization expressed in Escherichia coli BL21/DE3 using dopa as substrate
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Macrophage migration inhibitory factor (CHEMBL2085)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Advances and Insights for Small Molecule Inhibition of Macrophage Migration Inhibitory Factor.
Trivedi-Parmar V, Jorgensen WL.
J Med Chem (2018) 61 : 8104 -8119
PubMed 29812929 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.40 5.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3797437 1 5.62
CHEMBL51085 EBSELEN 3.0 1 5.62
CHEMBL496 HEXACHLOROPHENE 4.0 1 5.22
CHEMBL4211781 1 5.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3797437 IC50 = 2400.0 nM 5.62
CHEMBL51085 EBSELEN IC50 = 2400.0 nM 5.62
CHEMBL496 HEXACHLOROPHENE IC50 = 6000.0 nM 5.22
CHEMBL4211781 IC50 = 7080.0 nM 5.15