Assay Detail
Binding
CHEMBL4199305
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human MIF tautomerization expressed in Escherichia coli BL21/DE3 using HPP as substrate preincubated for 15 mins followed by substrate addition measured for 3 mins
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Macrophage migration inhibitory factor (CHEMBL2085) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Advances and Insights for Small Molecule Inhibition of Macrophage Migration Inhibitory Factor.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 4 | 5.84 | 6.24 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3797437 | — | — | 1 | 6.24 |
| CHEMBL51085 | EBSELEN | 3.0 | 1 | 6.24 |
| CHEMBL4211781 | — | — | 1 | 5.61 |
| CHEMBL496 | HEXACHLOROPHENE | 4.0 | 1 | 5.26 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3797437 | — | Ki | = | 570.0 | nM | 6.24 |
| CHEMBL51085 | EBSELEN | Ki | = | 570.0 | nM | 6.24 |
| CHEMBL4211781 | — | Ki | = | 2470.0 | nM | 5.61 |
| CHEMBL496 | HEXACHLOROPHENE | Ki | = | 5550.0 | nM | 5.26 |