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Assay Detail

CHEMBL4199570

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human RET using cisbio TK biotin-peptide as substrate preincubated for 30 mins followed by substrate addition measured after 1 hr by HTRF assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proto-oncogene tyrosine-protein kinase receptor Ret (CHEMBL2041)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a First-in-Class Gut-Restricted RET Kinase Inhibitor as a Clinical Candidate for the Treatment of IBS.
Schenck Eidam H, Russell J, Raha K, DeMartino M, Qin D, Guan HA, Zhang Z, Zhen G, Yu H, Wu C, Pan Y, Joberty G, Zinn N, Laquerre S, Robinson S, White A, Giddings A, Mohammadi E, Greenwood-Van Meerveld B, Oliff A, Kumar S, Cheung M.
ACS Med Chem Lett (2018) 9 : 623 -628
PubMed 30034590 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 9.15 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4218013 1 10.00
CHEMBL4218563 1 9.70
CHEMBL4218127 1 9.40
CHEMBL4205243 1 9.40
CHEMBL4212513 1 9.40
CHEMBL3355010 1 9.40
CHEMBL3355009 GSK-3179106 1.0 1 9.40
CHEMBL4203429 1 9.22
CHEMBL4205803 1 9.15
CHEMBL4214146 1 9.15
CHEMBL4213626 1 9.10
CHEMBL4210670 1 8.92
CHEMBL4206308 1 8.77
CHEMBL4209229 1 8.55
CHEMBL4215099 1 7.65

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4218013 IC50 = 0.1 nM 10.00
CHEMBL4218563 IC50 = 0.2 nM 9.70
CHEMBL4218127 IC50 = 0.4 nM 9.40
CHEMBL4205243 IC50 = 0.4 nM 9.40
CHEMBL4212513 IC50 = 0.4 nM 9.40
CHEMBL3355010 IC50 = 0.4 nM 9.40
CHEMBL3355009 GSK-3179106 IC50 = 0.4 nM 9.40
CHEMBL4203429 IC50 = 0.6 nM 9.22
CHEMBL4205803 IC50 = 0.7 nM 9.15
CHEMBL4214146 IC50 = 0.7 nM 9.15
CHEMBL4213626 IC50 = 0.8 nM 9.10
CHEMBL4210670 IC50 = 1.2 nM 8.92
CHEMBL4206308 IC50 = 1.7 nM 8.77
CHEMBL4209229 IC50 = 2.8 nM 8.55
CHEMBL4215099 IC50 = 22.2 nM 7.65