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Assay Detail

CHEMBL4199839

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human GST-tagged mTOR catalytic domain (1360 to 2549 residues) expressed in baculovirus expression system using GFP-4EBP1 as substrate measured after 1 hr by Lathascreen method
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase mTOR (CHEMBL2842)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of an Orally Bioavailable Dual PI3K/mTOR Inhibitor Based on Sulfonyl-Substituted Morpholinopyrimidines.
Shen S, He X, Yang Z, Zhang L, Liu Y, Zhang Z, Wang W, Liu W, Li Y, Huang D, Sun K, Ni X, Yang X, Chu X, Cui Y, Lv Q, Lan J, Zhou F.
ACS Med Chem Lett (2018) 9 : 719 -724
PubMed 30034607 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.75 6.73

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4211915 1 6.73
CHEMBL4215969 1 5.78
CHEMBL2017974 BUPARLISIB 3.0 1 5.70
CHEMBL4206442 1 5.67
CHEMBL4210320 1 4.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4211915 IC50 = 186.0 nM 6.73
CHEMBL4215969 IC50 = 1680.0 nM 5.78
CHEMBL2017974 BUPARLISIB IC50 = 1981.0 nM 5.70
CHEMBL4206442 IC50 = 2120.0 nM 5.67
CHEMBL4210320 IC50 = 13980.0 nM 4.85