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Assay Detail

CHEMBL4199918

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of IDH1 R132H mutant (unknown origin) using alphaKG as substrate in presence of NADPH by LC-MS/MS analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Isocitrate dehydrogenase [NADP] cytoplasmic (CHEMBL2007625)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of 3-Pyrimidin-4-yl-oxazolidin-2-ones as Orally Bioavailable and Brain Penetrant Mutant IDH1 Inhibitors.
Zhao Q, Manning JR, Sutton J, Costales A, Sendzik M, Shafer CM, Levell JR, Liu G, Caferro T, Cho YS, Palermo M, Chenail G, Dooley J, Villalba B, Farsidjani A, Chen J, Dodd S, Gould T, Liang G, Slocum K, Pu M, Firestone B, Growney J, Heimbach T, Pagliarini R.
ACS Med Chem Lett (2018) 9 : 746 -751
PubMed 30034612 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.44 7.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4214694 1 7.44
CHEMBL4216196 1 7.41
CHEMBL4207387 1 6.21
CHEMBL3682033 1 6.09
CHEMBL3677127 1 5.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4214694 IC50 = 36.0 nM 7.44
CHEMBL4216196 IC50 = 39.0 nM 7.41
CHEMBL4207387 IC50 = 610.0 nM 6.21
CHEMBL3682033 IC50 = 820.0 nM 6.09
CHEMBL3677127 IC50 = 8800.0 nM 5.06