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Assay Detail

CHEMBL4200147

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TNFalpha-stimulated NF-kappaB (unknown origin) expressed in HEK293 cells incubated for 30 mins followed by TNFalpha stimulation for 4 hrs by luciferase reporter gene based luminescence assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

NF-κB and Angiogenesis Inhibitors from the Aerial Parts of Chresta martii.
Queiroz MMF, Monteillier A, Berndt S, Marcourt L, Franco ES, Carpentier G, Nejad Ebrahimi S, Cuendet M, Bolzani VDS, Maia MBS, Queiroz EF, Wolfender JL.
J Nat Prod (2018) 81 : 1769 -1776
PubMed 30067035 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.53 6.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4205156 1 6.16
CHEMBL3322516 1 6.16
CHEMBL4213932 1 5.75
CHEMBL4210056 1 5.66
CHEMBL4218862 1 5.62
CHEMBL4209530 1 5.29
CHEMBL4214441 1 5.19
CHEMBL4205686 1 5.11
CHEMBL4217390 1 4.87

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4205156 IC50 = 700.0 nM 6.16
CHEMBL3322516 IC50 = 700.0 nM 6.16
CHEMBL4213932 IC50 = 1800.0 nM 5.75
CHEMBL4210056 IC50 = 2200.0 nM 5.66
CHEMBL4218862 IC50 = 2400.0 nM 5.62
CHEMBL4209530 IC50 = 5100.0 nM 5.29
CHEMBL4214441 IC50 = 6500.0 nM 5.19
CHEMBL4205686 IC50 = 7800.0 nM 5.11
CHEMBL4217390 IC50 = 13600.0 nM 4.87