Assay Detail
Functional
CHEMBL4201490
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Agonist activity at rat CB1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-induced cAMP accumulation incubated for 30 mins
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Rattus norvegicus |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
( R)- N-(1-Methyl-2-hydroxyethyl)-13-( S)-methyl-arachidonamide (AMG315): A Novel Chiral Potent Endocannabinoid Ligand with Stability to Metabolizing Enzymes.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 12 | 8.10 | 9.52 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4215369 | — | — | 1 | 9.52 |
| CHEMBL4208628 | — | — | 1 | 9.22 |
| CHEMBL4213900 | — | — | 1 | 8.72 |
| CHEMBL4208217 | — | — | 1 | 8.32 |
| CHEMBL4218304 | — | — | 1 | 7.98 |
| CHEMBL4216075 | — | — | 1 | 7.88 |
| CHEMBL4213566 | — | — | 1 | 7.55 |
| CHEMBL4206063 | — | — | 1 | 7.44 |
| CHEMBL4211106 | — | — | 1 | 7.23 |
| CHEMBL15848 | ANANDAMIDE | — | 1 | 7.16 |
| CHEMBL4207686 | — | — | 1 | - |
| CHEMBL4215534 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4215369 | — | EC50 | = | 0.3 | nM | 9.52 |
| CHEMBL4208628 | — | EC50 | = | 0.6 | nM | 9.22 |
| CHEMBL4213900 | — | EC50 | = | 1.9 | nM | 8.72 |
| CHEMBL4208217 | — | EC50 | = | 4.8 | nM | 8.32 |
| CHEMBL4218304 | — | EC50 | = | 10.4 | nM | 7.98 |
| CHEMBL4216075 | — | EC50 | = | 13.2 | nM | 7.88 |
| CHEMBL4213566 | — | EC50 | = | 28.4 | nM | 7.55 |
| CHEMBL4206063 | — | EC50 | = | 36.1 | nM | 7.44 |
| CHEMBL4211106 | — | EC50 | = | 58.3 | nM | 7.23 |
| CHEMBL15848 | ANANDAMIDE | EC50 | = | 69.0 | nM | 7.16 |
| CHEMBL4207686 | — | EC50 | - | — | - | |
| CHEMBL4215534 | — | EC50 | - | — | - |