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Assay Detail

CHEMBL4201511

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to BTN3A1 in human PBMC assessed as stimulation of Vgamma9 Vdelta2 T cells incubated for 3 days and measured after 11 days post compound removal by flow cytometry
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Butyrophilin subfamily 3 member A1 (CHEMBL4105758)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Phosphonamidate Prodrugs of a Butyrophilin Ligand Display Plasma Stability and Potent Vγ9 Vδ2 T Cell Stimulation.
Lentini NA, Foust BJ, Hsiao CC, Wiemer AJ, Wiemer DF.
J Med Chem (2018) 61 : 8658 -8669
PubMed 30199251 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 8.84 9.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4203452 1 9.47
CHEMBL4204044 1 9.44
CHEMBL4215122 1 9.44
CHEMBL4216254 1 9.36
CHEMBL145233 1 9.29
CHEMBL4215653 1 9.28
CHEMBL4210700 1 9.24
CHEMBL4207267 1 9.09
CHEMBL4204584 1 8.96
CHEMBL4208921 1 8.82
CHEMBL4088948 1 8.27
CHEMBL4172453 1 5.40
CHEMBL4202914 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4203452 EC50 = 0.34 nM 9.47
CHEMBL4204044 EC50 = 0.36 nM 9.44
CHEMBL4215122 EC50 = 0.36 nM 9.44
CHEMBL4216254 EC50 = 0.44 nM 9.36
CHEMBL145233 EC50 = 0.51 nM 9.29
CHEMBL4215653 EC50 = 0.53 nM 9.28
CHEMBL4210700 EC50 = 0.58 nM 9.24
CHEMBL4207267 EC50 = 0.82 nM 9.09
CHEMBL4204584 EC50 = 1.1 nM 8.96
CHEMBL4208921 EC50 = 1.5 nM 8.82
CHEMBL4088948 EC50 = 5.4 nM 8.27
CHEMBL4172453 EC50 = 4000.0 nM 5.40
CHEMBL4202914 EC50 > 10000.0 nM -