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Assay Detail

CHEMBL4201520

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to BTN3A1 in human Vgamma9 Vdelta2 T cells mixed with human K562 cells pretreated with compound for 4 hrs assessed as INF-gamma production measured after 20 hrs by ELISA
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Butyrophilin subfamily 3 member A1 (CHEMBL4105758)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Phosphonamidate Prodrugs of a Butyrophilin Ligand Display Plasma Stability and Potent Vγ9 Vδ2 T Cell Stimulation.
Lentini NA, Foust BJ, Hsiao CC, Wiemer AJ, Wiemer DF.
J Med Chem (2018) 61 : 8658 -8669
PubMed 30199251 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 9 6.72 7.21

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4215122 1 7.21
CHEMBL4215653 1 7.03
CHEMBL4210700 1 6.92
CHEMBL4216254 1 6.89
CHEMBL4204044 1 6.77
CHEMBL4207267 1 6.68
CHEMBL4203452 1 6.54
CHEMBL4208921 1 6.34
CHEMBL4204584 1 6.13

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4215122 EC50 = 62.0 nM 7.21
CHEMBL4215653 EC50 = 93.0 nM 7.03
CHEMBL4210700 EC50 = 120.0 nM 6.92
CHEMBL4216254 EC50 = 130.0 nM 6.89
CHEMBL4204044 EC50 = 170.0 nM 6.77
CHEMBL4207267 EC50 = 210.0 nM 6.68
CHEMBL4203452 EC50 = 290.0 nM 6.54
CHEMBL4208921 EC50 = 460.0 nM 6.34
CHEMBL4204584 EC50 = 740.0 nM 6.13