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Assay Detail

CHEMBL4201979

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human MDM2 (1 to 125 residues) and p53 (unknown origin) interaction pre-incubated for 20 mins before peptide-2-biot addition and measured after 3 hrs by HTRF assay
19
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Intermediate

Target

Tumour suppressor p53/oncoprotein Mdm2 (CHEMBL1907611)
Type PROTEIN-PROTEIN INTERACTION
Organism Homo sapiens

Publication

Inhibition of p53-Murine Double Minute 2 (MDM2) Interactions with 3,3'-Spirocyclopentene Oxindole Derivatives.
Gicquel M, Gomez C, Garcia Alvarez MC, Pamlard O, Guérineau V, Jacquet E, Bignon J, Voituriez A, Marinetti A.
J Med Chem (2018) 61 : 9386 -9392
PubMed 30221935 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 6.51 8.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4203529 2 8.51
CHEMBL191334 NUTLIN-3 1 8.46
CHEMBL4218748 1 8.00
CHEMBL4207959 2 7.62
CHEMBL4217905 1 7.48
CHEMBL4209483 2 6.52
CHEMBL4211645 1 6.46
CHEMBL4212066 1 6.21
CHEMBL4203887 2 6.18
CHEMBL4206333 2 6.15
CHEMBL4210216 1 6.13
CHEMBL4212404 1 5.90
CHEMBL4205549 1 5.47
CHEMBL4214609 1 5.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4203529 IC50 = 3.1 nM 8.51
CHEMBL191334 NUTLIN-3 IC50 = 3.5 nM 8.46
CHEMBL4218748 IC50 = 10.1 nM 8.00
CHEMBL4207959 IC50 = 23.7 nM 7.62
CHEMBL4217905 IC50 = 33.03 nM 7.48
CHEMBL4209483 IC50 = 303.5 nM 6.52
CHEMBL4211645 IC50 = 349.5 nM 6.46
CHEMBL4203529 IC50 = 538.2 nM 6.27
CHEMBL4212066 IC50 = 619.0 nM 6.21
CHEMBL4203887 IC50 = 654.8 nM 6.18
CHEMBL4206333 IC50 = 712.2 nM 6.15
CHEMBL4210216 IC50 = 738.8 nM 6.13
CHEMBL4203887 IC50 = 1039.0 nM 5.98
CHEMBL4207959 IC50 = 1144.0 nM 5.94
CHEMBL4212404 IC50 = 1257.0 nM 5.90
CHEMBL4206333 IC50 = 3032.5 nM 5.52
CHEMBL4205549 IC50 = 3384.5 nM 5.47
CHEMBL4209483 IC50 = 3724.7 nM 5.43
CHEMBL4214609 IC50 = 3680.0 nM 5.43