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Assay Detail

CHEMBL4202145

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human VMAT2 expressed in HEK293 cell membranes assessed as reduction in [3H[-5HT uptake pre-incubated for 10 mins before [3H[-5HT addition and measured after 6 mins
20
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Synaptic vesicular amine transporter (CHEMBL1893)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and Discovery of Arylpiperidinylquinazolines: New Inhibitors of the Vesicular Monoamine Transporter.
Provencher BA, Eshleman AJ, Johnson RA, Shi X, Kryatova O, Nelson J, Tian J, Gonzalez M, Meltzer PC, Janowsky A.
J Med Chem (2018) 61 : 9121 -9131
PubMed 30240563 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 6.67 7.88

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL772 RESERPINE 4.0 1 7.88
CHEMBL4205254 2 7.80
CHEMBL4207658 1 7.71
CHEMBL4206824 1 7.55
CHEMBL4202787 1 7.41
CHEMBL4214975 1 7.07
CHEMBL4208250 1 7.00
CHEMBL4208779 1 6.90
CHEMBL4213176 1 6.85
CHEMBL213814 1 6.81
CHEMBL51 KETANSERIN 3.0 1 6.75
CHEMBL39 SEROTONIN 3.0 1 5.84
CHEMBL4216117 1 5.57
CHEMBL4203894 1 5.54
CHEMBL508 1 5.44
CHEMBL122270 LOBELINE 2.0 1 5.38
CHEMBL4204449 1 5.24
CHEMBL4212633 1 -
CHEMBL4217581 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL772 RESERPINE IC50 = 13.2 nM 7.88
CHEMBL4205254 IC50 = 16.0 nM 7.80
CHEMBL4207658 IC50 = 19.7 nM 7.71
CHEMBL4206824 IC50 = 28.1 nM 7.55
CHEMBL4202787 IC50 = 39.4 nM 7.41
CHEMBL4205254 IC50 = 51.1 nM 7.29
CHEMBL4214975 IC50 = 85.5 nM 7.07
CHEMBL4208250 IC50 = 101.0 nM 7.00
CHEMBL4208779 IC50 = 125.0 nM 6.90
CHEMBL4213176 IC50 = 142.0 nM 6.85
CHEMBL213814 IC50 = 154.0 nM 6.81
CHEMBL51 KETANSERIN IC50 = 180.0 nM 6.75
CHEMBL39 SEROTONIN IC50 = 1430.0 nM 5.84
CHEMBL4216117 IC50 = 2700.0 nM 5.57
CHEMBL4203894 IC50 = 2900.0 nM 5.54
CHEMBL508 IC50 = 3620.0 nM 5.44
CHEMBL122270 LOBELINE IC50 = 4200.0 nM 5.38
CHEMBL4204449 IC50 = 5800.0 nM 5.24
CHEMBL4212633 IC50 > 10000.0 nM -
CHEMBL4217581 IC50 > 10000.0 nM -