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Assay Detail

CHEMBL4202146

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of VMAT2 in C57Bl/6J mouse striatal membranes assessed as reduction in [3H[-5HT uptake pre-incubated for 10 mins before [3H[-5HT addition and measured after 8 mins
20
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Tissue Striatum
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Synaptic vesicular amine transporter (CHEMBL4295886)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and Discovery of Arylpiperidinylquinazolines: New Inhibitors of the Vesicular Monoamine Transporter.
Provencher BA, Eshleman AJ, Johnson RA, Shi X, Kryatova O, Nelson J, Tian J, Gonzalez M, Meltzer PC, Janowsky A.
J Med Chem (2018) 61 : 9121 -9131
PubMed 30240563 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.56 8.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL772 RESERPINE 4.0 1 8.75
CHEMBL4205254 2 8.47
CHEMBL4202787 1 8.46
CHEMBL4207658 1 8.04
CHEMBL4208779 1 7.89
CHEMBL4206824 1 7.70
CHEMBL4214975 1 7.61
CHEMBL4208250 1 7.50
CHEMBL213814 1 7.30
CHEMBL39 SEROTONIN 3.0 1 7.01
CHEMBL51 KETANSERIN 3.0 1 7.00
CHEMBL122270 LOBELINE 2.0 1 6.42
CHEMBL508 1 5.56
CHEMBL4203894 1 -
CHEMBL4216117 1 -
CHEMBL4204449 1 -
CHEMBL4212633 1 -
CHEMBL4217581 1 -
CHEMBL4213176 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL772 RESERPINE IC50 = 1.79 nM 8.75
CHEMBL4205254 IC50 = 3.39 nM 8.47
CHEMBL4202787 IC50 = 3.46 nM 8.46
CHEMBL4205254 IC50 = 6.6 nM 8.18
CHEMBL4207658 IC50 = 9.1 nM 8.04
CHEMBL4208779 IC50 = 12.8 nM 7.89
CHEMBL4206824 IC50 = 19.8 nM 7.70
CHEMBL4214975 IC50 = 24.7 nM 7.61
CHEMBL4208250 IC50 = 31.5 nM 7.50
CHEMBL213814 IC50 = 49.56 nM 7.30
CHEMBL39 SEROTONIN IC50 = 98.0 nM 7.01
CHEMBL51 KETANSERIN IC50 = 100.0 nM 7.00
CHEMBL122270 LOBELINE IC50 = 381.0 nM 6.42
CHEMBL508 IC50 = 2740.0 nM 5.56
CHEMBL4203894 IC50 - -
CHEMBL4216117 IC50 - -
CHEMBL4204449 IC50 - -
CHEMBL4212633 IC50 - -
CHEMBL4217581 IC50 - -
CHEMBL4213176 IC50 - -