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Assay Detail

CHEMBL4222628

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Functional
Antagonist activity at human recombinant 5-HT2A receptor expressed in CHOK1 cells assessed as inhibition of serotonin-induced calcium release preincubated for 15 mins followed by 5-HT addition measured every 1.7 secs for 60 secs by calcium 4 dye-based FLIPR assay
16
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 2A (CHEMBL224)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and activity of functionalizable derivatives of the serotonin (5-HT) 5-HT2A receptor (5-HT2AR) antagonist M100907.
Gilbertson SR, Chen YC, Soto CA, Yang Y, Rice KC, Cunningham KA, Anastasio NC.
Bioorg Med Chem Lett (2018) 28 : 1381 -1385
PubMed 29555153 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 7.55 8.32
Inhibition 4 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL74355 VOLINANSERIN 3.0 3 8.32
CHEMBL4227912 2 7.53
CHEMBL4227059 3 7.44
CHEMBL4226407 2 7.40
CHEMBL4228313 3 7.33
CHEMBL4226858 3 7.25

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL74355 VOLINANSERIN IC50 = 4.786 nM 8.32
CHEMBL74355 VOLINANSERIN IC50 = 4.8 nM 8.32
CHEMBL4227912 IC50 = 29.51 nM 7.53
CHEMBL4227912 IC50 = 29.5 nM 7.53
CHEMBL4227059 IC50 = 36.31 nM 7.44
CHEMBL4227059 IC50 = 36.5 nM 7.44
CHEMBL4226407 IC50 = 39.81 nM 7.40
CHEMBL4226407 IC50 = 39.8 nM 7.40
CHEMBL4228313 IC50 = 46.77 nM 7.33
CHEMBL4228313 IC50 = 46.7 nM 7.33
CHEMBL4226858 IC50 = 56.23 nM 7.25
CHEMBL4226858 IC50 = 56.6 nM 7.25
CHEMBL74355 VOLINANSERIN Inhibition - % -
CHEMBL4228313 Inhibition - % -
CHEMBL4226858 Inhibition - % -
CHEMBL4227059 Inhibition - % -