Assay Detail
Binding
CHEMBL4223022
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant Neisseria meningitidis glycosyltransferase LgtC using UDP-Gal/lactose as substrate preincubated for 30 mins with UDP-Gal followed by lactose addition measured after 20 mins in presence of MnCl2 and calf intestinal phosphatase by malachite green dye based enzyme-coupled colorimetric assay relative to control
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Neisseria meningitidis |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Publication
Structure-activity relationships in a new class of non-substrate-like covalent inhibitors of the bacterial glycosyltransferase LgtC.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 5.10 | 5.54 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL379966 | — | — | 1 | 5.54 |
| CHEMBL4227320 | — | — | 1 | 5.52 |
| CHEMBL4060241 | — | — | 1 | 5.51 |
| CHEMBL4227590 | — | — | 1 | 5.37 |
| CHEMBL4226574 | — | — | 1 | 4.92 |
| CHEMBL4074318 | — | — | 1 | 4.75 |
| CHEMBL4229261 | — | — | 1 | 4.60 |
| CHEMBL4227242 | — | — | 1 | 4.55 |
| CHEMBL4228599 | — | — | 1 | - |
| CHEMBL4226164 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL379966 | — | IC50 | = | 2900.0 | nM | 5.54 |
| CHEMBL4227320 | — | IC50 | = | 3000.0 | nM | 5.52 |
| CHEMBL4060241 | — | IC50 | = | 3100.0 | nM | 5.51 |
| CHEMBL4227590 | — | IC50 | = | 4300.0 | nM | 5.37 |
| CHEMBL4226574 | — | IC50 | = | 12000.0 | nM | 4.92 |
| CHEMBL4074318 | — | IC50 | = | 18000.0 | nM | 4.75 |
| CHEMBL4229261 | — | IC50 | = | 25000.0 | nM | 4.60 |
| CHEMBL4227242 | — | IC50 | = | 28000.0 | nM | 4.55 |
| CHEMBL4228599 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL4226164 | — | IC50 | > | 100000.0 | nM | - |