Assay Detail
Binding
CHEMBL4223433
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to CA-SP1 P373S/V370A double mutant in HIV-1 subtype B NL4-3 infected in human MT2 cells assessed as inhibition of viral maturation by measuring virus yield after 4 to 5 days in presence of 10% FBS/27 mg/ml HSA/40% human serum by luciferase reporter gene assay
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Human immunodeficiency virus 1 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Intermediate |
Target
Gag-Pol polyprotein (CHEMBL3638360) ↗| Type | SINGLE PROTEIN |
| Organism | Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)(HIV-1) |
Publication
The design, synthesis and structure-activity relationships associated with C28 amine-based betulinic acid derivatives as inhibitors of HIV-1 maturation.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 1 | 7.82 | 7.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3827026 | — | — | 1 | 7.82 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3827026 | — | EC50 | = | 15.0 | nM | 7.82 |