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Assay Detail

CHEMBL4223780

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TGase 2 in human A375 cells using R-I-Cad/DMC as substrate preincubated for 5 mins followed by substrate addition measured at 30 secs interval over 900 secs by fluorescence anisotropy assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein-glutamine gamma-glutamyltransferase (CHEMBL2730)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Nε-Acryloyllysine Piperazides as Irreversible Inhibitors of Transglutaminase 2: Synthesis, Structure-Activity Relationships, and Pharmacokinetic Profiling.
Wodtke R, Hauser C, Ruiz-Gómez G, Jäckel E, Bauer D, Lohse M, Wong A, Pufe J, Ludwig FA, Fischer S, Hauser S, Greif D, Pisabarro MT, Pietzsch J, Pietsch M, Löser R.
J Med Chem (2018) 61 : 4528 -4560
PubMed 29664627 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 6.15 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4227301 1 6.40
CHEMBL2203451 1 6.10
CHEMBL4228168 1 5.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4227301 IC50 = 400.0 nM 6.40
CHEMBL2203451 IC50 = 790.0 nM 6.10
CHEMBL4228168 IC50 = 1100.0 nM 5.96