Assay Detail
Binding
CHEMBL4231814
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant N-terminal GST-tagged human AKR1C2 expressed in Escherichia coli BL21 (DE) Codon Plus RP cells using S-tetralol as substrate in presence of NADP+ by fluorimetric analysis
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Aldo-keto reductase family 1 member C2 (CHEMBL5847) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Potent and selective aldo-keto reductase 1C3 (AKR1C3) inhibitors based on the benzoisoxazole moiety: application of a bioisosteric scaffold hopping approach to flufenamic acid.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 4.97 | 6.28 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL23588 | FLUFENAMIC ACID | 2.0 | 1 | 6.28 |
| CHEMBL4249650 | — | — | 1 | 5.37 |
| CHEMBL4245231 | — | — | 1 | 5.36 |
| CHEMBL4249427 | — | — | 1 | 5.24 |
| CHEMBL4248154 | — | — | 1 | 4.26 |
| CHEMBL4238730 | — | — | 1 | 4.17 |
| CHEMBL4174786 | — | — | 1 | 4.13 |
| CHEMBL4238142 | — | — | 1 | - |
| CHEMBL4238437 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL23588 | FLUFENAMIC ACID | IC50 | = | 530.0 | nM | 6.28 |
| CHEMBL4249650 | — | IC50 | = | 4290.0 | nM | 5.37 |
| CHEMBL4245231 | — | IC50 | = | 4380.0 | nM | 5.36 |
| CHEMBL4249427 | — | IC50 | = | 5700.0 | nM | 5.24 |
| CHEMBL4248154 | — | IC50 | = | 54870.0 | nM | 4.26 |
| CHEMBL4238730 | — | IC50 | = | 66950.0 | nM | 4.17 |
| CHEMBL4174786 | — | IC50 | = | 73230.0 | nM | 4.13 |
| CHEMBL4238142 | — | IC50 | = | 108130.0 | nM | - |
| CHEMBL4238437 | — | IC50 | = | 118550.0 | nM | - |