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Assay Detail

CHEMBL4233455

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HDAC1 (unknown origin) using Ac-peptide as substrate pretreated for 15 mins followed by substrate addition and measured after 1 hr by fluorescence assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 1 (CHEMBL325)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and anticancer evaluation of acridine hydroxamic acid derivatives as dual Topo and HDAC inhibitors.
Chen J, Li D, Li W, Yin J, Zhang Y, Yuan Z, Gao C, Liu F, Jiang Y.
Bioorg Med Chem (2018) 26 : 3958 -3966
PubMed 29954683 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.52 8.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4241141 1 8.41
CHEMBL4240694 1 8.29
CHEMBL4237410 1 8.09
CHEMBL4251261 1 7.85
CHEMBL98 VORINOSTAT 4.0 1 7.84
CHEMBL4248226 1 7.18
CHEMBL4248676 1 6.87
CHEMBL4244419 1 6.65
CHEMBL4244887 1 6.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4241141 IC50 = 3.9 nM 8.41
CHEMBL4240694 IC50 = 5.1 nM 8.29
CHEMBL4237410 IC50 = 8.2 nM 8.09
CHEMBL4251261 IC50 = 14.1 nM 7.85
CHEMBL98 VORINOSTAT IC50 = 14.3 nM 7.84
CHEMBL4248226 IC50 = 66.2 nM 7.18
CHEMBL4248676 IC50 = 133.8 nM 6.87
CHEMBL4244419 IC50 = 224.3 nM 6.65
CHEMBL4244887 IC50 = 306.2 nM 6.51