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Assay Detail

CHEMBL4236606

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal truncated recombinant human LSD1 (151 to 852 residues) expressed in Escherichia coli using histone H3 (1 to 21 residues) K4me2 peptide as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by peroxidase-coupled assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific histone demethylase 1A (CHEMBL6136)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of 5-arylidene barbiturates as potent, selective, reversible LSD1 inhibitors for the treatment of acute promyelocytic leukemia.
Xu S, Zhou C, Liu R, Zhu Q, Xu Y, Lan F, Zha X.
Bioorg Med Chem (2018) 26 : 4871 -4880
PubMed 30153955 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 5.78 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3651585 1 8.10
CHEMBL4248408 1 6.52
CHEMBL4240336 1 6.39
CHEMBL4241311 1 6.26
CHEMBL4245069 1 6.06
CHEMBL4240782 1 5.96
CHEMBL4244605 1 5.92
CHEMBL4239151 1 5.55
CHEMBL4238393 1 5.51
CHEMBL4244132 1 5.44
CHEMBL4251434 1 5.44
CHEMBL257990 (+)-TRANYLCYPROMINE 1 5.44
CHEMBL4237686 1 5.36
CHEMBL4237521 1 5.19
CHEMBL4248955 1 4.74
CHEMBL4248864 1 4.64
CHEMBL4250459 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3651585 IC50 = 8.0 nM 8.10
CHEMBL4248408 IC50 = 300.0 nM 6.52
CHEMBL4240336 IC50 = 410.0 nM 6.39
CHEMBL4241311 IC50 = 550.0 nM 6.26
CHEMBL4245069 IC50 = 870.0 nM 6.06
CHEMBL4240782 IC50 = 1100.0 nM 5.96
CHEMBL4244605 IC50 = 1200.0 nM 5.92
CHEMBL4239151 IC50 = 2800.0 nM 5.55
CHEMBL4238393 IC50 = 3100.0 nM 5.51
CHEMBL4244132 IC50 = 3600.0 nM 5.44
CHEMBL4251434 IC50 = 3600.0 nM 5.44
CHEMBL257990 (+)-TRANYLCYPROMINE IC50 = 3600.0 nM 5.44
CHEMBL4237686 IC50 = 4400.0 nM 5.36
CHEMBL4237521 IC50 = 6420.0 nM 5.19
CHEMBL4248955 IC50 = 18200.0 nM 4.74
CHEMBL4248864 IC50 = 23000.0 nM 4.64
CHEMBL4250459 IC50 > 75000.0 nM -