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Assay Detail

CHEMBL4251843

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-c-AMP from His-Tb-tagged human PDE4D2 (86 to 413 residues) preincubated for 10 mins followed by [3H]-c-AMP addition measured after 1 hr by scintillation proximity assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

3',5'-cyclic-AMP phosphodiesterase 4D (CHEMBL288)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of imidazo[1,2-<i>b</i>]pyridazine TYK2 pseudokinase ligands as potent and selective allosteric inhibitors of TYK2 signalling.
Moslin R, Gardner D, Santella J, Zhang Y, Duncia JV, Liu C, Lin J, Tokarski JS, Strnad J, Pedicord D, Chen J, Blat Y, Zupa-Fernandez A, Cheng L, Sun H, Chaudhry C, Huang C, D'Arienzo C, Sack JS, Muckelbauer JK, Chang C, Tredup J, Xie D, Aranibar N, Burke JR, Carter PH, Weinstein DS.
Medchemcomm (2017) 8 : 700 -712
PubMed 30108788 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.14 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4293907 1 8.22
CHEMBL4291200 1 7.89
CHEMBL4289426 1 7.82
CHEMBL4278757 1 7.75
CHEMBL4285841 1 7.62
CHEMBL4287761 1 7.50
CHEMBL4279883 1 7.37
CHEMBL4279157 1 7.21
CHEMBL4295039 1 7.06
CHEMBL4291611 1 6.42
CHEMBL4283351 1 5.62
CHEMBL4290130 1 5.25
CHEMBL4281304 1 -
CHEMBL4293510 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4293907 IC50 = 6.0 nM 8.22
CHEMBL4291200 IC50 = 13.0 nM 7.89
CHEMBL4289426 IC50 = 15.0 nM 7.82
CHEMBL4278757 IC50 = 18.0 nM 7.75
CHEMBL4285841 IC50 = 24.0 nM 7.62
CHEMBL4287761 IC50 = 32.0 nM 7.50
CHEMBL4279883 IC50 = 43.0 nM 7.37
CHEMBL4279157 IC50 = 62.0 nM 7.21
CHEMBL4295039 IC50 = 88.0 nM 7.06
CHEMBL4291611 IC50 = 380.0 nM 6.42
CHEMBL4283351 IC50 = 2400.0 nM 5.62
CHEMBL4290130 IC50 = 5600.0 nM 5.25
CHEMBL4281304 IC50 > 100000.0 nM -
CHEMBL4293510 IC50 > 33000.0 nM -