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Assay Detail

CHEMBL4253389

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CDK4/Cyclin-D1 (unknown origin) using histone-H1 as substrate after 90 mins by ADP-Glo assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Intermediate

Target

Cyclin-dependent kinase 4/cyclin D1 (CHEMBL1907601)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

A highly potent CDK4/6 inhibitor was rationally designed to overcome blood brain barrier in gliobastoma therapy.
Yin L, Li H, Liu W, Yao Z, Cheng Z, Zhang H, Zou H.
Eur J Med Chem (2018) 144 : 1 -28
PubMed 29247857 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 7.89 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4288327 1 8.52
CHEMBL4277900 CROZBACICLIB 2.0 1 8.52
CHEMBL4276993 1 8.40
CHEMBL4284941 1 8.40
CHEMBL4288165 1 8.22
CHEMBL4281514 1 8.22
CHEMBL4279791 1 8.10
CHEMBL4277525 1 8.10
CHEMBL4285830 1 8.10
CHEMBL4292139 1 8.00
CHEMBL4289350 1 7.92
CHEMBL4284768 1 7.89
CHEMBL4294940 1 7.82
CHEMBL4284301 1 7.80
CHEMBL4280895 1 7.80
CHEMBL4287743 1 7.77
CHEMBL4286100 1 7.75
CHEMBL4279275 1 7.75
CHEMBL4288134 1 7.55
CHEMBL4282981 1 7.48
CHEMBL4284737 1 7.46
CHEMBL4289967 1 7.38
CHEMBL4280423 1 7.36
CHEMBL4283885 1 7.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4288327 IC50 = 3.0 nM 8.52
CHEMBL4277900 CROZBACICLIB IC50 = 3.0 nM 8.52
CHEMBL4276993 IC50 = 4.0 nM 8.40
CHEMBL4284941 IC50 = 4.0 nM 8.40
CHEMBL4288165 IC50 = 6.0 nM 8.22
CHEMBL4281514 IC50 = 6.0 nM 8.22
CHEMBL4279791 IC50 = 8.0 nM 8.10
CHEMBL4277525 IC50 = 8.0 nM 8.10
CHEMBL4285830 IC50 = 8.0 nM 8.10
CHEMBL4292139 IC50 = 10.0 nM 8.00
CHEMBL4289350 IC50 = 12.0 nM 7.92
CHEMBL4284768 IC50 = 13.0 nM 7.89
CHEMBL4294940 IC50 = 15.0 nM 7.82
CHEMBL4284301 IC50 = 16.0 nM 7.80
CHEMBL4280895 IC50 = 16.0 nM 7.80
CHEMBL4287743 IC50 = 17.0 nM 7.77
CHEMBL4286100 IC50 = 18.0 nM 7.75
CHEMBL4279275 IC50 = 18.0 nM 7.75
CHEMBL4288134 IC50 = 28.0 nM 7.55
CHEMBL4282981 IC50 = 33.0 nM 7.48
CHEMBL4284737 IC50 = 35.0 nM 7.46
CHEMBL4289967 IC50 = 42.0 nM 7.38
CHEMBL4280423 IC50 = 44.0 nM 7.36
CHEMBL4283885 IC50 = 78.0 nM 7.11