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Assay Detail

CHEMBL4253672

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-tagged LSD1 (2 to 852 residues) (unknown origin) expressed in baculovirus infected sf9 cells preincubated for 15 mins followed by H3K4me2 substrate addition measured after 60 mins by amplex red reagent based HRP enzyme coupled fluorescence assay
48
Total Activities
46
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific histone demethylase 1A (CHEMBL6136)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-activity studies on N-Substituted tranylcypromine derivatives lead to selective inhibitors of lysine specific demethylase 1 (LSD1) and potent inducers of leukemic cell differentiation.
Schulz-Fincke J, Hau M, Barth J, Robaa D, Willmann D, Kürner A, Haas J, Greve G, Haydn T, Fulda S, Lübbert M, Lüdeke S, Berg T, Sippl W, Schüle R, Jung M.
Eur J Med Chem (2018) 144 : 52 -67
PubMed 29247860 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 48 6.86 7.45

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4285212 1 7.45
CHEMBL4281778 2 7.29
CHEMBL4292597 1 7.22
CHEMBL4277281 1 7.19
CHEMBL4290752 1 7.18
CHEMBL4285166 1 7.18
CHEMBL4287709 1 7.18
CHEMBL4292825 1 7.16
CHEMBL4289408 2 7.11
CHEMBL4278920 1 7.10
CHEMBL4284179 1 7.09
CHEMBL4279808 1 7.08
CHEMBL4282845 1 7.04
CHEMBL4287563 1 7.04
CHEMBL4285989 1 7.02
CHEMBL4294148 1 7.02
CHEMBL4285786 1 7.00
CHEMBL4288523 1 7.00
CHEMBL4278738 1 6.98
CHEMBL4285695 1 6.96
CHEMBL4294062 1 6.96
CHEMBL4284077 1 6.95
CHEMBL4289655 1 6.95
CHEMBL4277841 1 6.94
CHEMBL4282381 1 6.93
CHEMBL4286163 1 6.93
CHEMBL4284708 1 6.92
CHEMBL4282608 1 6.91
CHEMBL4280740 1 6.83
CHEMBL4282324 1 6.81

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4285212 IC50 = 35.3 nM 7.45
CHEMBL4281778 IC50 = 51.8 nM 7.29
CHEMBL4292597 IC50 = 60.0 nM 7.22
CHEMBL4277281 IC50 = 64.5 nM 7.19
CHEMBL4290752 IC50 = 65.5 nM 7.18
CHEMBL4285166 IC50 = 66.1 nM 7.18
CHEMBL4287709 IC50 = 66.5 nM 7.18
CHEMBL4292825 IC50 = 69.1 nM 7.16
CHEMBL4289408 IC50 = 77.5 nM 7.11
CHEMBL4278920 IC50 = 79.7 nM 7.10
CHEMBL4284179 IC50 = 80.7 nM 7.09
CHEMBL4279808 IC50 = 82.6 nM 7.08
CHEMBL4287563 IC50 = 91.0 nM 7.04
CHEMBL4282845 IC50 = 91.1 nM 7.04
CHEMBL4294148 IC50 = 95.9 nM 7.02
CHEMBL4285989 IC50 = 94.9 nM 7.02
CHEMBL4289408 IC50 = 95.6 nM 7.02
CHEMBL4288523 IC50 = 100.2 nM 7.00
CHEMBL4281778 IC50 = 99.5 nM 7.00
CHEMBL4285786 IC50 = 100.0 nM 7.00
CHEMBL4278738 IC50 = 103.9 nM 6.98
CHEMBL4294062 IC50 = 108.5 nM 6.96
CHEMBL4285695 IC50 = 110.0 nM 6.96
CHEMBL4284077 IC50 = 112.1 nM 6.95
CHEMBL4289655 IC50 = 111.7 nM 6.95
CHEMBL4277841 IC50 = 114.1 nM 6.94
CHEMBL4282381 IC50 = 117.0 nM 6.93
CHEMBL4286163 IC50 = 117.5 nM 6.93
CHEMBL4284708 IC50 = 119.9 nM 6.92
CHEMBL4282608 IC50 = 122.0 nM 6.91
CHEMBL4280740 IC50 = 146.5 nM 6.83
CHEMBL4282324 IC50 = 154.5 nM 6.81
CHEMBL4292976 IC50 = 159.0 nM 6.80
CHEMBL4277206 IC50 = 157.9 nM 6.80
CHEMBL4288999 IC50 = 187.3 nM 6.73
CHEMBL4294740 IC50 = 194.6 nM 6.71
CHEMBL4292356 IC50 = 202.4 nM 6.69
CHEMBL4290483 IC50 = 202.8 nM 6.69
CHEMBL4287919 IC50 = 276.5 nM 6.56
CHEMBL4289595 IC50 = 294.5 nM 6.53
CHEMBL492697 IC50 = 321.1 nM 6.49
CHEMBL4282210 IC50 = 325.0 nM 6.49
CHEMBL4292813 IC50 = 417.5 nM 6.38
CHEMBL4278320 IC50 = 643.5 nM 6.19
CHEMBL4284789 IC50 = 817.1 nM 6.09
CHEMBL257990 (+)-TRANYLCYPROMINE IC50 = 6500.0 nM 5.19
CHEMBL4284358 IC50 - -
CHEMBL4283975 IC50 - -